Abstract
A partially purified potato polyphenol oxidase (PPO) was immobilized in a cross-linked chitosan–SiO2 gel and used to treat phenol solutions. Under optimized conditions (formaldehyde 20 mg/ml, PPO 4 mg/ml and pH 7.0), the activity of immobilized PPO was 1370 U/g and its K m value for catechol was 12 mm at 25°C. The highest activity of immobilized enzyme was at pH 7.4. Immobilization stabilized the enzyme with 73 and 58% retention of activity after 10 and 20 days, respectively, at 30°C whereas most of the free enzyme was inactive after 7 days. The efficiency of removing phenol (10 mg phenol/l) by the immobilized PPO was 86%, and about 60% removal efficiency was retained after five recycles. The immobilized PPO may thus be a useful for removing phenolic compounds from industrial waste-waters.
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Acknowledgements
The financial supports of the National Natural Science Foundation of China (grant number: 30540063) and the Nature Science Foundation of Jiangsu Province, P.R. China (grant Number: BK2005042; BK2005202) are gratefully acknowledged.
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Shao, J., Ge, H. & Yang, Y. Immobilization of polyphenol oxidase on chitosan–SiO2 gel for removal of aqueous phenol. Biotechnol Lett 29, 901–905 (2007). https://doi.org/10.1007/s10529-007-9329-2
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DOI: https://doi.org/10.1007/s10529-007-9329-2