Abstract
A phosphate starvation-induced acid phosphatase cDNA was cloned from the rice, Oryza sativa. The cDNA encoding O. sativa acid phosphatase (OsACP1) has 1100 bp with an open reading frame of 274 amino acid residues. The deduced amino acid sequence of OsACP1 cDNA showed 53% identity to tomato acid phosphatase and 46–50% identity to several other plant phosphatases. OsACP1 expression was up-regulated in the rice plant and in cell culture in the absence of phosphate (Pi). The induced expression of OsACP1 was a specific response to Pi starvation, and was not affected by the deprivation of other nutrients. OsACP1 expression was responsive to the level of Pi supply, with transcripts of OsACP1 being abundant in Pi-deprived root. The OsACP1 cDNA was expressed as a 30 kDa polypeptide in baculovirus-infected insect Sf9 cells. In addition, the OsACP1 gene was introduced into Arabidopsis via Agrobacterium-mediated transformation. Functional expression of the OsACP1 gene in the transgenic Arabidopsis lines was confirmed by Northern blot and Western blot analyses, as well as phosphatase activity assays. These results suggest that the OsACP1 gene can be used to develop new transgenic dicotyledonous plants able to adapt to Pi-deficient conditions.
Similar content being viewed by others
References
Baldwin JC, Karthikeyan AS, Raghothama KG (2001) LEPS2, a Phosphorus Starvation-Induced Novel Acid Phosphatase from Tomato. Plant Physiol 125:728–737
Bosse D, Kock M (1998) Influence of phosphate starvation on phosphohydrolases during development of tomato seedlings. Plant Cell Environ 21:325–332
Clough SJ, Bent AF (1998) Floral dip: a simplified method for Agrobacterium mediated transformation of Arabidopsis thaliana. Plant J 16:735–743
Collet JF, Stroobant V, Pirard M, Delpierre G, Van Schaftingen E (1998) A new class of phosphotransferases phosphorylated on an aspartate residue in an aminoterminal DXDX(T/V) motif. J Biol Chem 273:14107–14112
del Pozo JC, Allona I, Rubio V, Leyva A, de la Pena A, Aragoncillo C, Paz-Ares J (1999) A type 5 acid phosphatase gene from Arabidopsis thalianais induced by phosphate starvation and by some other types of phosphate mobilising/oxidative stress conditions. Plant J 19:579–589
Duff SMG, Gautam S, Plaxton WC (1994) The role of acid phosphatases in plant phosphorus metabolism. Physiol Plant 90:791–800
Goldstein AH, Baertlein DA, McDaniel RG (1988) Phosphate starvation inducible metabolism in lycopersicon esculentum I. Excretion of acid phosphatase by tomato plants and suspension-cultured cells. Plant Physiol 87:711–715
Hubel F, Beck E (1996) Maize root phytase: purification, characterization, and localization of enzyme activity and its putative substrate. Plant Physiol 112:1429–1436
Je YH, Chang JH, Choi JY, Roh JY, Jin BR, O’Reilly DR, Kang SK (2001) A defective viral genome maintained in Escherichia coli for the generation of baculovirus expression vectors. Biotechnol Lett 23:575–582
Miller SS, Liu J, Allan DL, Menzhuber CJ, Fedorova M, Vance CP (2001) Molecular control of acid phosphatase secretion into the rhizosphere of proteoid roots from phosphorus-stressed white lupin. Plant Physiol 127:594–606
O’Reilly DR, Miller LK, Luckow VA (1992) Baculovirus expression vectors: a laboratory manual. W. H. Freeman & Co., New York
Raghothama KG (1999) Phosphate acquisition. Annu Rev Plant Physiol Plant Mol Biol 50:665–693
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The ClustalX windows interface : flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24:4876–4882
Yu SM, Kuo YH, Sheu G, Sheu YJ, Liu LF (1991) Metabolic derepression of alpha-amylase gene expression in suspension-cultured cells of rice. J Biol Chem 266:21131–21137
Acknowledgements
This work was supported by a grant from the Crop Functional Genomics Center of the 21st Century Frontier Research Program (No. CG1716) and the Biogreen 21 programs, Rural Development Administration (RDA), Republic of Korea.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Hur, Y.J., Lee, H.G., Jeon, E.J. et al. A phosphate starvation-induced acid phosphatase from Oryza sativa: phosphate regulation and transgenic expression . Biotechnol Lett 29, 829–835 (2007). https://doi.org/10.1007/s10529-007-9318-5
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10529-007-9318-5