Abstract
Mineralization of diuron has not been previously demonstrated despite the availability of some bacteria to degrade diuron into 3,4-dichloroaniline (3,4-DCA) and others that can mineralize 3,4-DCA. A bacterial co-culture of Arthrobacter sp. N4 and Delftia acidovorans W34, which respectively degraded diuron (20 mg l−1) to 3,4-DCA and mineralized 3,4-DCA, were able to mineralize diuron. Total diuron mineralization (20 mg l−1) was achieved with free cells in co-culture. When the bacteria were immobilized (either one bacteria or both), the degradation rate was higher. Best results were obtained with free Arthrobacter sp. N4 cells co-cultivated with immobilized cells of D. acidovorans W34 (mineralization of diuron in 96 h, i.e., 0.21 mg l−1 h−1 vs. 0.06 mg l−1 h−1 with free cells in co-culture).
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Acknowledgement
We are very grateful to Jerome Kuntz, assistant engineer (Plate-Forme Technologique AGROSYSTEMES), for his technical assistance and to Martine Sancelme and Winnie Dejonghe for providing us strains of Arthrobacter sp. N4 and D. acidovorans W34, respectively.
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Bazot, S., Bois, P., Joyeux, C. et al. Mineralization of diuron [3-(3,4-dichlorophenyl)-1, 1-dimethylurea] by co-immobilized Arthrobacter sp. and Delftia acidovorans . Biotechnol Lett 29, 749–754 (2007). https://doi.org/10.1007/s10529-007-9316-7
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DOI: https://doi.org/10.1007/s10529-007-9316-7