Abstract
A mutan-degrading bacterium, closely related to Paenibacillus curdlanolyticus, was isolated from soil. It produced 0.4 U mutanase ml−1 in 2 days in shake-flask cultures when bacterial mutan was the sole carbon source. Mutanase activity was optimal at pH 6.2 and 45°C over 1 h and was stable between pH 5.8 and 12 at 4°C for 24 h and up to 40°C for 1 h. Mutan produced by Streptococcus mutans was rapidly hydrolyzed by this enzyme. The hydrolysis of mutan (1 g l−1) resulted in 17% saccharification over 2 h and, at the same time, glucan was entirely solubilized.
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This work was financially supported by the BW/BS/BiNoZ/UMCS Research Programs.
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Pleszczyńska, M., Marek-Kozaczuk, M., Wiater, A. et al. Paenibacillus strain MP-1: a new source of mutanase. Biotechnol Lett 29, 755–759 (2007). https://doi.org/10.1007/s10529-007-9311-z
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DOI: https://doi.org/10.1007/s10529-007-9311-z