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Regioselective and enzymatic production of γ-resorcylic acid from resorcinol using recombinant Escherichia coli cells expressing a novel decarboxylase gene

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Abstract

A recombinant Escherichia coli, expressing the rdc gene, which encodes a γ-resorcylic acid decarboxylase (Rdc) reversibly catalyzing regioselective carboxylation of resorcinol derived from Rhizobium radiobacter WU-0108, converted 20 mM resorcinol to γ-resorcylic acid with a 44% (mol/mol) yield at 30°C for 7 h. The recombinant E. coli cells were recyclable at least five times for use as biocatalysts.

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Acknowledgements

This work was partly supported by the 21COE program “Practical Nano-Chemistry” from MEXT, Japan.

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Authors and Affiliations

  1. Department of Applied Chemistry, School of Science and Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku-ku, Tokyo, 169-8555, Japan

    Yuichiro Iwasaki, Kuniki Kino, Hiroyuki Nishide & Kohtaro Kirimura

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  1. Yuichiro Iwasaki
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  2. Kuniki Kino
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  3. Hiroyuki Nishide
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  4. Kohtaro Kirimura
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Correspondence to Kohtaro Kirimura.

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Iwasaki, Y., Kino, K., Nishide, H. et al. Regioselective and enzymatic production of γ-resorcylic acid from resorcinol using recombinant Escherichia coli cells expressing a novel decarboxylase gene. Biotechnol Lett 29, 819–822 (2007). https://doi.org/10.1007/s10529-007-9309-6

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  • DOI: https://doi.org/10.1007/s10529-007-9309-6

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