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Biosynthesis of intracellular 5-aminolevulinic acid by a newly identified halotolerant Rhodobacter sphaeroides

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Abstract

Of 23 strains of halotolerant (up to 12% w/v NaCl) photosynthetic bacteria isolated from various sources, one isolate, SH5, accumulated intracellular 5-aminolevulinic acid (ALA) at 0.45 μg/g dry cell wt (DCW) growing aerobically in the dark. The strain was identified as Rhodobacter sphaeroides using 16S rDNA sequencing. Biosynthesis of ALA was enhanced to 14 μg/g DCW using modified glutamate/glucose (50 mM) medium with the addition of 10 mM levulinic acid after 24 h cultivation. Addition of 30 μM Fe2+ to this medium increased the yield to 226 μg/g DCW.

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Acknowledgements

The authors would like to express their gratitude to The Royal Golden Jubilee (RGJ) Ph.D. Program for the Scholarship (PHD/0087/2542) and Basic Research Grant (BGJ) under Thailand Research Fund (TRF) and Prince of Songkla University for financial support and thanks to Professor Dr. Horst W. Doelle for his valuable comments and corrections during preparation of this manuscript.

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Authors and Affiliations

  1. Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand

    Amornrat Tangprasittipap & Poonsuk Prasertsan

  2. Biotechnology Program, School of Agricultural Technology, Walailak University, Nakhornsithammarat, 80160, Thailand

    Wanna Choorit

  3. Faculty of Engineering, Hiroshima Kokusai Gakuin University, Hiroshima, 793-0321, Japan

    Ken Sasaki

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  1. Amornrat Tangprasittipap
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  2. Poonsuk Prasertsan
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  3. Wanna Choorit
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  4. Ken Sasaki
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Correspondence to Poonsuk Prasertsan.

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Tangprasittipap, A., Prasertsan, P., Choorit, W. et al. Biosynthesis of intracellular 5-aminolevulinic acid by a newly identified halotolerant Rhodobacter sphaeroides . Biotechnol Lett 29, 773–778 (2007). https://doi.org/10.1007/s10529-006-9303-4

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  • DOI: https://doi.org/10.1007/s10529-006-9303-4

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