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Development of microsatellite markers from tartary buckwheat

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Abstract

A genomic library enriched with (gT)n repeats from tartary buckwheat (Fagopyrum tataricum) was constructed using 5′-anchored PCR for the development of microsattellite markers. Sequencing analysis of 5 clones from the library showed that they all contained microsatellites (totally 10 loci), and each was unique. An additional locus-specific primer was designed according to flanking sequence. Two of the microsatellite loci of 10 tartary buckwheat varieties were amplified using an anchored primer and a locus-specific primer, which revealed a clear polymorphic pattern. The data confirmed that the degenerate primer was reliably anchoring at the 5′-end of the microsatellite, and the primers developed based on this technology could be used for diversity analysis of tartary buckwheat.

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Acknowledgments

This work was supported by The program of International Plant Genetic Resources Institute (LOAAPO04074) and programs of Science and Technology of Shanxi province, China(041147, 2006031047).

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Authors and Affiliations

  1. Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan, 030006, P. R. China

    Yan-Qin Li & Tong-Lin Shi

  2. IPGRI Office for East Asia, c/o The Chinese Academy of Agricultural Sciences, Beijing, 100081, P. R. China

    Zong-Wen Zhang

Authors
  1. Yan-Qin Li
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  2. Tong-Lin Shi
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  3. Zong-Wen Zhang
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Correspondence to Yan-Qin Li.

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Li, YQ., Shi, TL. & Zhang, ZW. Development of microsatellite markers from tartary buckwheat. Biotechnol Lett 29, 823–827 (2007). https://doi.org/10.1007/s10529-006-9293-2

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  • DOI: https://doi.org/10.1007/s10529-006-9293-2

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