Abstract
Crystal violet (CV), which has been extensively used as a biological stain and a commercial textile dye, is a recalcitrant molecule. A strain of Pseudomonas putida was isolated that effectively degraded CV: up to 80% of 60 μM CV as the sole carbon source, was degraded in liquid media within 1 week. Nine degradation products were isolated and identified. We propose that CV degradation occurs via a stepwise demethylation process to yield mono-, di-, tri-, tetra-, penta- and hexa-demethylated CV species.



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Chen, CC., Liao, HJ., Cheng, CY. et al. Biodegradation of crystal violet by Pseudomonas putida . Biotechnol Lett 29, 391–396 (2007). https://doi.org/10.1007/s10529-006-9265-6
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DOI: https://doi.org/10.1007/s10529-006-9265-6

