Biotechnology Letters

, Volume 29, Issue 3, pp 431–437

A novel high-copy plasmid, pEC, compatible with commonly used Escherichia coli cloning and expression vectors

Original research paper

DOI: 10.1007/s10529-006-9258-5

Cite this article as:
Zhang, H., Wu, H. & Zhang, H. Biotechnol Lett (2007) 29: 431. doi:10.1007/s10529-006-9258-5

Abstract

A 66164-bp cryptic plasmid, pEIB1, was isolated from strain Vibrio anguillarum MVM425 and sequenced. A plasmid carrying a 1089-bp fragment, containing the minimal replication region of pEIB1, a kanamycin-resistance marker and an l-arabinose promoter, designated pEC, was maintained as a high copy plasmid in E. coli and stably inherited in the absence of antibiotic selection. Significantly, pEC was compatible with the widely used ColE1, pSC101 and p15A replicons making it a useful tool for a dual-plasmid expression system.

Keywords

Plasmid vector pEC Compatible origin Copy number Stability Dual plasmids expression Gene regulation 

Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  1. 1.State Key Laboratory of Bioreactor Engineering (Mailbox 431)East China University of Science and TechnologyShanghaiChina

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