Enzymatic synthesis of (R)-cyanohydrins by a novel (R)-oxynitrilase from Vicia sativa L.
- 198 Downloads
The defatted seed meal of common vetch (Vicia sativa L.) served as a source of (R)-oxynitrilase which catalyzed the enantioselective addition of HCN to aromatic, heteroaromatic, fluoro-substituted aromatic aldehydes to produce the corresponding enantiomeric pure cyanohydrins in yields of 52–100% and 88–99% ee at 12°C under micro-aqueous medium (diisopropyl ether) without addition of buffer solution.
KeywordsBiotransformation Common vetch Cyanohydrin Oxynitrilase Vicia sativa L.
- Zandbergen P, van der Linden J, Brussee J, van der Gen A (1991) Synthesis of optically active cyanohydrins using almond meal. Synth Commun 21:1387–1391Google Scholar