Abstract
Transposon Tn5 mutagenesis was used to generate random mutations in Shewanella baltica MAC1, a polyunsaturated fatty acid (PUFA)-producing bacterium. Three mutants produced 3–5 times more eicosapentaenoic acid (EPA 20:5 n−3) compared to the wild type at 10°C. One of the mutants produced 0.3 mg EPA g−1 when grown at high temperature (30°C). Moreover, 2 mg docosahexaenoic acid (DHA 22:6 n−3) g−1 was produced by S. baltica mutants at 4°C. Sequencing of insertion mutation(s) showed 96% homology to trimethylamine N-oxide (TMAO) reductase gene and 85% homology to rRNA operons of E. coli. Tn5 transposon mutagenesis therefore is a suitable technique to increase PUFA formation in bacteria.
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Acknowledgements
We thank Dr. Swift for providing the pUT Mini-Tn5 Km2 plasmid. This work was supported by grants from Dairy Farmers of Ontario and the Natural Sciences and Engineering Research Council of Canada.
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Amiri-Jami, M., Wang, H., Kakuda, Y. et al. Enhancement of Polyunsaturated Fatty Acid Production by Tn5 Transposon in Shewanella baltica . Biotechnol Lett 28, 1187–1192 (2006). https://doi.org/10.1007/s10529-006-9077-8
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DOI: https://doi.org/10.1007/s10529-006-9077-8