Abstract
Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (Apo2L/TRAIL) is a new member of the TNF superfamily. In this work, the key role of Zn2+ in high-level expression of soluble TRAIL was confirmed. The yield of soluble TRAIL reached 1.6 g l−1 using a novel, two-stage Zn2+ feeding strategy, and the accumulation of TRAIL inclusion bodies decreased. Furthermore, the purified TRAIL showed stronger cytotoxicity activity against human pancreatic 1990 tumor cells as the molar ratio of Zn2+ to TRAIL monomer was 2 in purified TRAIL solution.
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This work was supported by a grant from the Ministry of Science and Technology (Key Project of the National High Technology Research and Development Program of China (863 Program): No. 2002AA2Z345A)
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Sun, AY., Shen, YL., Yin, JC. et al. Improvement of Expression Level and Bioactivity of Soluble Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (Apo2L/TRAIL) by a Novel Zinc ion Feeding Strategy. Biotechnol Lett 28, 1215–1219 (2006). https://doi.org/10.1007/s10529-006-9073-z
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DOI: https://doi.org/10.1007/s10529-006-9073-z