Abstract
Ethephon at 50 µM enhanced both root growth and ginsenoside accumulation in ginseng (Panax ginseng C.A. Meyer) adventitious root cultures, but at 100 µM it inhibited only ginsenoside accumulation. Ginsenoside productivity with 50 µM ethephon was the highest at 1.7 mg l−1 d−1 after 8 days of elicitation. However, elicitation with 50 µM ethephon and 100 µM methyl jasmonate (MJ) improved productivity (6.3 mg l−1 d−1) whereas elicitation with 100 µM MJ alone gave only 2.9 mg l−1 d−1.
Similar content being viewed by others
References
Cho GH, Kim DI, Pedersen H, Chin CK (1988) Ethephon enhancement of secondary metabolite synthesis in plant cell cultures. Biotechnol Prog 4:184–188
Hahn EJ, Kim YS, Yu KW, Jeong CS, Paek KY (2003) Adventitious root cultures of Panax ginseng C.A. Meyer and ginsenoside production through large-scale bioreactor system. J Plant Biotechnol 5:1–6
Haralampidis K, Trojanowska M, Osbourn AE (2002) Biosynthesis of triterpenoid saponins in plants. Adv Biochem Eng Biotechnol 75:31–49
Jeong CS, Chakrabarty D, Hahn EJ, Lee HL, Paek KY (2006) Effect of oxygen, carbon dioxide and ethylene on growth and bioactive compound production in bioreactor culture of ginseng adventitious roots. Biochem Eng J 27:252–263
Kim YS, Hahn EJ, Murthy HN, Paek KY (2004) Adventitious root growth and ginsenoside accumulation in Panax ginseng cultures as affected by methyl jasmonate. Biotechnol Lett 26:1619–1622
Pan ZW, Wang HQ, Zhong JJ (2000) Scale-up study on suspension cultures of Taxus chinensis cells for production of taxane diterpene. Enzyme Microb Technol 27:714–723
Wu J, Zhong JJ (1999) Production of ginseng and its bioactive components in plant cell culture: current technology and applied aspects. J Biotechnol 68:89–99
Xiaojie X, Xiangyang H, Neill SJ, Jianying F, Weiming C (2005) Fungal elicitor induces singlet oxygen generation, ethylene release and saponin synthesis in cultured cells of Panax ginseng C.A. Meyer. Plant Cell Physiol 46:947–954
Yu KW, Gao W, Hahn EJ, Paek KY (2002) Jasmonic acid improves ginsenoside accumulation in adventitious root culture of Panax ginseng C.A. Meyer. Biochem Eng J 11:211–215
Zhao J, Davis LC, Verpoorte R (2005) Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnol Adv 23:283–333
Acknowledgement
This work supported by Korean Research Foundation Grant (KRF−2003–005-F00003).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Bae, KH., Choi, YE., Shin, CG. et al. Enhanced Ginsenoside Productivity by Combination of Ethephon and Methyl Jasmoante in Ginseng (Panax ginseng C.A. Meyer) Adventitious Root Cultures. Biotechnol Lett 28, 1163–1166 (2006). https://doi.org/10.1007/s10529-006-9071-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10529-006-9071-1