Abstract
A new growth-based screening method for the identification of enantioselective hydrolases, such as lipases and esterases, using pro-antibiotic substrates was devised. An enantioselective hydrolase could be identified by measuring growth rates of cells in liquid media containing (R)- or (S)-2–phenylbutyric chloramphenicol esters. This method can be applied to the screening of novel enantioselective microbes and to the high-throughput screening for the directed evolution of enantioselective hydrolytic enzymes.
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This research was partially supported by the Nano Bioelectronics and Systems Research Center by the Korean Science and Engineering Foundation (KOSEF).
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Hwang, BY., Oh, JM., Kim, J. et al. Pro-antibiotic Substrates for the Identification of Enantioselective Hydrolases. Biotechnol Lett 28, 1181–1185 (2006). https://doi.org/10.1007/s10529-006-9069-8
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DOI: https://doi.org/10.1007/s10529-006-9069-8