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Response of Growth and Fatty Acid Compositions of Nannochloropsis sp. to Environmental Factors Under Elevated CO2 Concentration

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Nannochloropsis sp. was grown with different levels of nitrate, phosphate, salinity and temperature with CO2 at 2,800 μl l−1. Increased levels of NaNO3 and KH2PO4 raised protein and polyunsaturated fatty acids (PUFAs) contents but decreased carbohydrate, total lipid and total fatty acids (TFA) contents. Nannochloropsis sp. grew well at salinities from 22 to 49 g l−1, and lowering salinity enhanced TFA and PUFAs contents. TFA contents increased with the increasing temperature but PUFAs contents decreased. The highest eicosapentaenoic acid (EPA, 20:5ω3) content based on the dry mass was above 3% under low N (150 μM NaNO3) or high N (3000 μM NaNO3) condition. Excessive nitrate, low salinity and temperature are thus favorable factors for improving EPA yields in Nannochloropsis sp.

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  • Al-Hasan RH, Ali AM, Ka’wash HH, Radwan SS (1990) Effect of salinity on the lipid and fatty acid composition of the halophyte Navicula sp.: potential in mariculture. J Appl Phycol 2:215–222

    Article  Google Scholar 

  • Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917

    PubMed  CAS  Google Scholar 

  • Chini Zittelli G, Lavista F, Bastianini A, Rodolfi L, Vincenzini M, Tredici MR (1999) Production of eicosapentaenoic acid by Nannochloropsis sp. cultures in outdoor tubular photobioreactors. J Biotech 70:299–312

    Article  CAS  Google Scholar 

  • Harrison PJ, Thompson PA, Calderwood GS (1990) Effects of nutrient and light limitation on the biochemical composition of phytoplankton. J Appl Phycol 2:45–56

    Article  Google Scholar 

  • Harrison PJ, Waters RE, Taylor FJR (1980) A broad spectrum artificial seawater medium for coastal and open ocean phytoplankton. J Phycol 16:28–35

    Google Scholar 

  • Hoshida H, Ohira T, Minematsu A, Akada R, Nishizawa Y (2005) Accumulation of eicosapentaenoic acid in Nannochloropsis sp. in response to elevated CO2 concentrations. J Appl Phycol 17:29–34

    Article  Google Scholar 

  • Hu H, Gao K (2003) Optimization of growth and fatty acid composition of a unicellular marine picoplankton, Nannochloropsis sp., with enriched carbon sources. Biotech Lett 25:421–425

    Article  CAS  Google Scholar 

  • James CM, Al-Hinty S, Salman AE (1989) Growth and ω3 fatty acid and amino acid composition of microalgae under different temperature regimes. Aquaculture 77:337–351

    Article  CAS  Google Scholar 

  • Kochert G (1978) Carbohydrate determination by the phenol–sulfuric acid method. In: Hellebust JA, Craigie JS (eds) Handbook of phycological methods: physiological and biochemical methods. Cambridge University Press, Cambridge, pp 95–97

    Google Scholar 

  • Lee Y-K, Tan H-M, Low C-S (1989) Effect of salinity of medium on cellular fatty acid composition of marine alga Porphyridium cruentum (Rhodophyceae). J Appl Phycol 1:19–23

    Article  CAS  Google Scholar 

  • Renaud SM, Parry DL (1994) Microalgae for use in tropical aquaculture II: effect of salinity on growth, gross chemical composition and fatty acid composition of three species of marine microalgae. J Appl Phycol 6:347–356

    Article  CAS  Google Scholar 

  • Renaud SM, Zhou HC, Parry DL, Thinh L-V, Woo KC (1995) Effect of temperature on the growth, total lipid content and fatty acid composition of recently isolated tropical microalgae Isochrysis sp., Nitzschia closterium, Nitzschia paleacea, and commercial species Isochrysis sp. (clone T.ISO). J Appl Phycol 7:595–602

    Article  CAS  Google Scholar 

  • Shifrin NS, Chisholm SW (1981) Phytoplankton lipids: interspecific differences and effects of nitrate, silicate and light-dark cycles. J Phycol 17:374–384

    Article  CAS  Google Scholar 

  • Sukenik A (1999) Production of eicosapentaenoic acid by the marine eustigmatophyte Nannochloropsis. In: Cohen Z (ed) Chemicals from microalgae. Taylor & Francis, London pp 41–56

    Google Scholar 

  • Uriarte I, Farías A, Hawkins AJS, Bayne BL (1993) Cell characteristics and biochemical composition of Dunaliella primolecta Butcher conditioned at different concentrations of dissolved nitrogen. J Appl Phycol 5:447–453

    Article  CAS  Google Scholar 

  • Xu N, Zhang X, Fan X, Han L, Zeng C (2001) Effects of nitrogen source and concentration on growth rate and fatty acid composition of Ellipsoidion sp. (Eustigmatophyta). J Appl Phycol 13:463–469

    Article  CAS  Google Scholar 

  • Zhu CJ, Lee YK, Chao TM (1997) Effect of temperature and growth phase on lipid and biochemical composition of Isochrysis galbana TK1. J Appl Phycol 9:451–457

    Article  CAS  Google Scholar 

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This study was funded by the National Natural Science Foundation of China (No. 90411018 and No. 30270036) and by the Chinese Academy of Sciences. The authors are grateful to Professor Ruixiang Li for providing the species.

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Correspondence to Hanhua Hu.

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Hu, H., Gao, K. Response of Growth and Fatty Acid Compositions of Nannochloropsis sp. to Environmental Factors Under Elevated CO2 Concentration. Biotechnol Lett 28, 987–992 (2006).

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