Biotechnology Letters

, Volume 28, Issue 11, pp 837–841 | Cite as

Anti-hepatitis B activities of ganoderic acid from Ganoderma lucidum

  • Yan-Qun LiEmail author
  • Shun-Fa Wang
Original paper


Ganoderic acid, from Ganoderma lucidum, at 8 µg/ml inhibited replication of hepatitis B virus (HBV) in HepG2215 cells over 8 days. Production of HBV surface antigen and HBV e antigen were 20 and 44% of controls without ganoderic acid. Male KM mice were significantly protected from liver injury, induced with carbon tetrachloride, by treatment with ganoderic acid at 10 mg and 30 mg/kg·d (by intravenous injection) 7 days. Ganoderic acid at the same dosage also significantly protected the mice from liver injury induced by M. bovis BCG plus lipopolysaccharide (from Escherichia coli 0127:B8).


Ganoderic acid Ganoderma lucidum Hepatitis Hepatoprotection Virus 


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  1. Back SJ, Jung WT, Kim DY, Cho KD (1999) G009: Hepatoprotectant, immunostimulant. Drugs Future 24:1068–1071CrossRefGoogle Scholar
  2. Hoder M, Wilkinson JH (1980) Enzymes. In: Brown SS, Mitchel FL, Young SD (eds) Chemical diagnosis of disease, 2nd edn. Biomed Press, New YorkGoogle Scholar
  3. Kim YS, Eo SK, Oh KW, Lee Ck, Han SS (2000) Antiherpetic activities of acidic protein bound polysaccharide isolated from Ganoderma lucidum alone and in combinations with interferons. J Ethnopharmacol 72:451–458PubMedCrossRefGoogle Scholar
  4. Li CH, Chen PY, Chang UM, Kan LS, Fang WH, Tsai KS, Lin SB (2005) Ganoderic acid X, a lanostanoid triterpene, inhibits topoisomerases and induces apoptosis of cancer cells. Life Sci 77:252–265PubMedCrossRefGoogle Scholar
  5. Min BS, Nakamura N, Hattori M (1998) Triterpenes from the spores of Ganoderma lucidum and their inhibitory activity against HIV-1 protease. Chem Pharm Bull 46:1607–1612PubMedGoogle Scholar
  6. Min BS, Gao JJ, Nakamura N, Hattori M (2000) Triterpenes from the spores of Ganoderma lucidum and their cytotoxicity against meth-A and LLC tumor cells. Chem Pharm Bull 48:1026–1033PubMedGoogle Scholar
  7. Min BS, Gao JJ, Hattori M, Lei HK, Kim YH (2001) Anticomplement activity of terpenoids from the spores of Ganoderma lucidum. Planta Med 67:811–814PubMedCrossRefGoogle Scholar
  8. Mizushina Y, Takahashi N, Hanashima L, Koshino H, Esumi Y, Uzawa J, Sugawara F, Sakaguchi K (1999) Lucidenic acid O and lactone, new terpene inhibitors of eukaryotic DNA polymerases from a basidiomycete, Ganoderma lucidum. Bioorg Med Chem 7:2047–2052PubMedCrossRefGoogle Scholar
  9. Oh KW, Lee CK, Kim YS, Eo SK, Han SS (2000) Antiherpetic activities of acidic protein bound polysacchride isolated from Ganoderma lucidum alone and in combinations with acyclovir and vidarabine. J Ethnopharmacol 72:221–227PubMedCrossRefGoogle Scholar
  10. Recknagel RO (1967) Carbon tetrachloride hepatotoxicity. Pharmacol Res 19:145–208Google Scholar
  11. Rees KR, Spector WG (1961) Reversible nature of liver cell damage due to carbon tetrachloride as demonstrate by the use of Phenergan. Nature (London) 189:821–829CrossRefGoogle Scholar
  12. Sahar EM, Meselhy MR, Nakamura N, Tezuka Y, Hattori M, Kakiuchi N, Shimotohno K, Kawahata T, Otake T (1998) Anti-Hiv-1 and anti-Hiv-1-protease substances from Ganoderma lucidum. Phytochemistry 49:1651–1657CrossRefGoogle Scholar
  13. Sells MA, Chen ML, Acs G (1987) Production of hepatitis B virus particles in HepG2 cells transfected with cloned hepatitis B virus DNA. Proc Natl Acad Sci USA 84:1005–1009PubMedCrossRefGoogle Scholar
  14. Sinha KP, Saran A (1972) Serum transaminase levels during the course of administration of carbon tetrachloride to rabbits. Ind J Med Res 60:1378–1381Google Scholar
  15. Su CY, Shiao MS, Wang CT (1999) Differential effects of ganodermic acid S on the thromboxane A2-signaling pathways in human platelets. Biochem Pharmacol 58:587–595PubMedCrossRefGoogle Scholar
  16. Tsujikura Y, Higuchi T, Miyamoto Y, Sato S (1992) Manufacture of ganoderic acid by fermentation of Ganoderma lucidum. Japanese Patent. JP04304890Google Scholar
  17. Wang SY, Hsu HH, Tzeng CH, Lee SS, Shiao MS, Ho CK (1997) The antitumor effect of Ganoderma lucidum is mediated by cytokines released from activated macrophages and T lymphocytes. Int J Cancer 70:699–705PubMedCrossRefGoogle Scholar
  18. Zhang JS, Tang QJ, Martin ZK, Reutter W, Fan H (2002) Activation of B lymphocytes by GLIS, a bioactive proteoglycan from Ganoderma lucidum. Life Sci 71:623–638PubMedCrossRefGoogle Scholar
  19. Zhao HB, Wang SZ, He Qh, Yuan L, Chen AF, Lin ZB (2005) Ganoderma total sterol (GS) and GS1 protect rat cerebral cortical neurons from hypoxia/reoxygenation injury. Life Sci 76:1027–1037PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  1. 1.College of Life ScienceSouth China Normal UniversityGuangzhouP.R. China
  2. 2.Jiangxi Academy of ScienceNanchangP.R. China

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