Skip to main content
SpringerLink
Log in

Properties of Laccases Produced by Pycnoporus sanguineus Induced by 2,5-xylidine

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Two isoforms of laccase produced from the culture supernatant of Pycnoporus sanguineus were partially purified by phenyl-Sepharose chromatography. Molecular masses of the enzymes were 80 kDa (Lac I) and 68 kDa (Lac II). Optimum activity of Lac I was at pH 4.8 and 30 °C, and Lac II was at pH 4.2 and 50 °C over 5 min reaction. The K m values of enzymes toward syringaldazine were 10 μm (Lac I) and 8 μm (Lac II). Sodium azide inhibited Lac I (85%) and Lac II (75%) activities.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • MT Cambria A Cambria S Ragusa E Rizzarelli (2000) ArticleTitleProduction, purification and properties of an extracellular laccase from Rigidoporus lignosus Protein Expres. Purif. 18 141–147 Occurrence Handle10.1006/prep.1999.1126 Occurrence Handle1:CAS:528:DC%2BD3cXht1Omsro%3D

    Article  CAS  Google Scholar 

  • N Das TK Chakraborty M Mukherjee (2001) ArticleTitlePurification and characterization of a growth-regulation laccase from Pleurotus florida J. Basic Microbiol. 41 261–267 Occurrence Handle10.1002/1521-4028(200110)41:5<261::AID-JOBM261>3.0.CO;2-V Occurrence Handle11688212 Occurrence Handle1:CAS:528:DC%2BD3MXotVyqsbg%3D

    Article  PubMed  CAS  Google Scholar 

  • C Eggert U Temp KE Eriksson (1996) ArticleTitleThe ligninolytic system of the white rot fungus Pycnoporus cinnabarinus: purification and characterization of the laccase Appl. Environ. Microbiol. 62 1151–1158 Occurrence Handle8919775 Occurrence Handle1:CAS:528:DyaK28XitFeksL0%3D

    PubMed  CAS  Google Scholar 

  • MLFC Gonçalves W Steiner (1996) ArticleTitleDetection of laccase activity in polyacrylamide gels after electrophoresis under denaturing conditions Biotech. Tech. 10 667–668

    Google Scholar 

  • JM Harkin JR Obst (1973) ArticleTitleSyringaldazine, an effective reagent for detecting laccase and peroxidases in fungi Experientia 29 IssueID4 381–387 Occurrence Handle10.1007/BF01926734 Occurrence Handle1:CAS:528:DyaE3sXktl2qt7Y%3D

    Article  CAS  Google Scholar 

  • G Hublick F Schinner (2000) ArticleTitleCharacterization and immobilization of the laccase from Pleurotus ostreatus and its use for the continuous elimination of phenolic pollutants Enz. Microbial Technol. 27 330–336 Occurrence Handle10.1016/S0141-0229(00)00220-9

    Article  Google Scholar 

  • H Jung F Xu K Li (2002) ArticleTitlePurification and characterization of laccase from wood-degrading fungus Trichophyton rubrum LKY-7 Enzyme Microbial Technol. 30 161–168 Occurrence Handle10.1016/S0141-0229(01)00485-9 Occurrence Handle1:CAS:528:DC%2BD38Xht1ant7s%3D

    Article  CAS  Google Scholar 

  • A Leonowicz NS Cho J Luterek A Wilkolazka M Wojtas-Wasilewska A Matuszewska M Hofrichter D Wesernberg J Rogalski (2001) ArticleTitleFungal laccase: properties and activity on lignin J. Basic Microbiol. 41 185–227 Occurrence Handle10.1002/1521-4028(200107)41:3/4<185::AID-JOBM185>3.0.CO;2-T Occurrence Handle11512451 Occurrence Handle1:CAS:528:DC%2BD3MXlvVynsr0%3D

    Article  PubMed  CAS  Google Scholar 

  • AM Mayer RC Staples (2002) ArticleTitleLaccase: new functions for an old enzyme Phytochemistry 60 551–565 Occurrence Handle10.1016/S0031-9422(02)00171-1 Occurrence Handle12126701 Occurrence Handle1:CAS:528:DC%2BD38Xlt1Ois7w%3D

    Article  PubMed  CAS  Google Scholar 

  • FH Périe GVB Reddy NJ Blackburn MH Gold (1998) ArticleTitlePurification and characterization of laccases from the white-rot basidiomycete Dichomitus squalens Arch. Biochem. Biophys. 353 349–355 Occurrence Handle9606969 Occurrence Handle10.1006/abbi.1998.0625

    Article  PubMed  Google Scholar 

  • SB Pointing EBG Jones LLP Vrijmoed (2000) ArticleTitleOptimization of laccase production by Pycnoporus sanguineus in submerged liquid culture Mycologia 92 139–144 Occurrence Handle1:CAS:528:DC%2BD3cXhvFCksb0%3D

    CAS  Google Scholar 

  • M Sugumaran (1995) ArticleTitleA caution about azide inhibition of enzymes associated with electrophilic metabolites Biochem. Biophys. Res. Commun. 212 834–839 Occurrence Handle10.1006/bbrc.1995.2044 Occurrence Handle7626118 Occurrence Handle1:CAS:528:DyaK2MXntFymtrY%3D

    Article  PubMed  CAS  Google Scholar 

  • CF Thurston (1994) ArticleTitleThe structure and function of fungal laccases Microbiology 140 19–26 Occurrence Handle1:CAS:528:DyaK2cXkt12lsrs%3D Occurrence Handle10.1099/13500872-140-1-19

    Article  CAS  Google Scholar 

  • S Vikineswary A Noorlidah M Renuvathani M Sekaran A Pandey EBG Jones (2006) ArticleTitleProductivity of laccase in solid substrate fermentation of selected agro-residues by Pycnoporus sanguineus Biores. Technol. 97 171–177 Occurrence Handle10.1016/j.biortech.2005.02.015 Occurrence Handle1:CAS:528:DC%2BD2MXpvVSktLw%3D

    Article  CAS  Google Scholar 

  • YZ Xiao Q Chen J Hang YY Shi J Wu YZ Hong YP Wang (2004) ArticleTitleSelective induction, purification and characterization of a laccase isoenzyme from the basidiomycete Trametes sp. AH28-2 Mycologia 96 26–35 Occurrence Handle1:CAS:528:DC%2BD2cXislOhu7c%3D

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Instituto Ciências Biológicas, Faculdade de Farmácia, Universidade Federal de Goiás, 74001-970, Goiânia, GO, Brazil

    Telma Alves Garcia, Mariângela Fontes Santiago & Cirano José Ulhoa

  2. Instituto de Biologia, Universidade de Brasília, Campus Asa Norte, 70.910-900, Brasília, DF, Brazil

    Telma Alves Garcia

Authors
  1. Telma Alves Garcia
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Mariângela Fontes Santiago
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Cirano José Ulhoa
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Cirano José Ulhoa.

Additional information

Revisions requested 30 November 2005; Revisions received 26 January 2006

Rights and permissions

Reprints and permissions

About this article

Cite this article

Garcia, T.A., Santiago, M.F. & Ulhoa, C.J. Properties of Laccases Produced by Pycnoporus sanguineus Induced by 2,5-xylidine. Biotechnol Lett 28, 633–636 (2006). https://doi.org/10.1007/s10529-006-0026-3

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-006-0026-3

Key words

Search

Navigation