Biotechnology Letters

, Volume 28, Issue 7, pp 497–503 | Cite as

Aluminium Chloride Enhances Colchicine Production in Root Cultures of Gloriosa superba



Root cultures of Gloriosa superba were treated with 5 mm methyl jasmonate and 125 μm AlCl3 which enhanced the intracellular colchicine content of the roots by 50-fold and 63-fold, respectively. Ten millimolar of CaCl2 and 1 mm CdCl2 enhanced biomass significantly (7- to 8.6-fold, respectively) while maximum release of colchicine into the medium was obtained with 10 mm CdCl2. Casein hydrolysate, yeast extract and silver nitrate had no significant effect on growth and colchicine accumulation in root cultures.


abiotic and biotic elicitors colchicine Gloriosa superba root culture 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Brodelius, P, Pedersen, H 1994Increasing secondary metabolite production in plant cell culture by redirecting transportTrends Biotechnol.113036Google Scholar
  2. Buitelaar, RM, Cesario, MT, Tramper, J 1992Elicitation of thiophene production by hairy roots of Tagetes patulaEnzyme Microb. Technol.1427CrossRefGoogle Scholar
  3. Chen, H, Chen, F 2000Effect of yeast elicitor on the secondary metabolism of Ti transformed Salvia miltiorrhiza cell suspension culturesPlant Cell Rep.19710717CrossRefGoogle Scholar
  4. Chen, H, Chen, F, Chiu Francis, CK, Lo Cindy, MY 2001The effect of yeast elicitor on the growth and secondary metabolism of hairy root cultures of Salvia miltiorrhizaEnzyme Microb. Technol.28100105CrossRefPubMedGoogle Scholar
  5. Ertola, RJ, Hours, R 1998Role of yeast extract components in microbial cultures not associated with amino acids, vitamins and minerals: a reviewAppl. Biol. Sci.27192201Google Scholar
  6. Finnie, JF, Staden, J 1991Isolation of colchicine from Sandersonia aurantiaca and Gloriosa superba. Variation in alkaloid levels of plants grown in vivoJ. Plant Physiol.138691695Google Scholar
  7. Finnie JF, Van Staden J (1994) Gloriosa superba L. (Flame Lily): micropropagation and in vitro production of colchicine. In: Bajaj YPS, ed. Biotechnology in Agriculture and Forestry, Vol. 26. Medicinal and Aromatic Plants VI, Chapter-X, Berlin: Springer-Verlag, pp. 146–166Google Scholar
  8. Furmanowa, M, Glowniak, K, Syklowska-Baranek, K, Zgórka, G, Józefczyk, A 1997Effect of picloram and methyl jasmonate on growth and taxane accumulation in callus culture of Taxus×media var. HatfieldiiPlant Cells Tissue Org. Cult.497579Google Scholar
  9. Furze, MJ, Rhodes, MJC, Parr, AJ, Robins, RJ, Whitehead, IA, Threfall, DR 1991Abiotic factors elicit sesquiterpenoid production but not alkaloid production in transformed root␣cultures of Datura stramoniumPlant Cell Rep.10111114CrossRefGoogle Scholar
  10. Ghosh, B, Mukherjee, M, Jha, TB, Jha, S 2002Enhanced colchicine production in root cultures of Gloriosa superba by direct and indirect precursors of the biosynthetic pathwayBiotechnol. Lett.24231234Google Scholar
  11. Gontier, E, Sangwan, BS, Barbotin, JN 1994Effects of calcium, alginate, and calcium alginate immobilization on growth and tropane alkaloid levels of a stable suspension cell line of Datura innoxia MillPlant Cell Rep.13533536CrossRefGoogle Scholar
  12. Hamel, F, Breton, Ch, Houde, M 1998Isolation and characterisation of wheat aluminium-regulated genes: possible involvement of aluminium as a pathogenesis response elicitorPlanta205531538CrossRefPubMedGoogle Scholar
  13. Hayashi, T, Yoshida, K, Sano, K 1988Formation of alkaloids in suspension cultured Colchicum autumnalePhytochemistry27371374Google Scholar
  14. Ketchum, REB, Tandon, M, Begley, TP, Gibson, DM, Croteau, R, Shuler, ML 1997The production of paclitaxel and other taxanes in Taxus canadensis suspension cell cultures elicited with methyl jasmonatePlant Physiol.114232Google Scholar
  15. Lang, B, Maturova, M, Reichstein, T, Šantavý, F 1959Isolation of substances from Gloriosa superbaPlanta Med.7298309Google Scholar
  16. Leslie, CA, Romani, RJ 1986Salicylic acid: a new inhibitor of ethylene biosynthesisPlant Cell Rep.5144146CrossRefGoogle Scholar
  17. Mukherjee, S, Ghosh, B, Jha, S 2000Enhanced forskolin production in genetically transformed cultures of Coleus forskohlii by casein hydrolysate and studies on growth and organizationBiotechnol. Lett.22133136CrossRefGoogle Scholar
  18. Murashige, T, Skoog, F 1962A revised medium for rapid growth and bioassays with tobacco tissue culturesPhysiol. Plant.15473497Google Scholar
  19. Nishi, A 1994

    Effect of elicitors on the production of secondary metabolites

    Ryu, DDYFurasaki, S eds. Advances in Plant BiotechnologyElsevier Science PublishersAmsterdam135151
    Google Scholar
  20. Pitta-Alvarez, SI, Spollansky, TC, Giulietti, AM 2000aThe influence of different biotic and abiotic elicitors on the production and profile of tropane alkaloids in hairy root cultures of Brugmansia candidaEnzyme Microb. Technol.26252258Google Scholar
  21. Pitta-Alvarez, SI, Spollansky, TC, Giulietti, AM 2000bScopolamine and hyoscyamine production by hairy root cultures of Brugmansia candida: influence of calcium chloride, hemicellulase and theophyllineBiotechnol. Lett.2216531656CrossRefGoogle Scholar
  22. Šantavý, F, Zajiček, DV, Něměcková, A 1957Isolation and identification of compounds from some plants related to Colchicum autumnaleCollection Czech. Chem. Commun.2214821488Google Scholar
  23. Sivakumar, G, Krishnamurthy, KV, Hahn, EJ, Paek, KY 2004Enhanced in vitro production of colchicine in Gloriosa superba L. – an emerging industrial medicinal crop in South IndiaJ. Horticult. Sci. Biotechnol.79602605Google Scholar
  24. Spollansky, TC, Pitta-Alvarez, SI, Giulietti, AM 2000Effect of jasmonic acid and aluminium on production of tropane alkaloids in hairy root cultures of Brugmansia candidaElectronic Journal of Biotechnology37275Google Scholar
  25. Suzuki, T, Mori, H, Yamame, T, Shimuzu, S 1985Automatich supplementation of minerals in fed-batch culture to high cell mass concentrationBiotech. Bioeng.27192201CrossRefGoogle Scholar
  26. Szabo, E, Thelen, A, Peterson, M 1999Fungal elicitor preparations and methyl jasmonate enhance rosmarinic acid accumulation in suspension cultures of Coleus blumeiPlant Cell Rep.18485489CrossRefGoogle Scholar
  27. Vázquez-Flota, F, Moreno-Valenzuela, O, Miranda-Ham, ML, Coello-Coello, J, Loyola-Vargas, VM 1994Catharanthine and ajmalicine synthesis in Catharanthus roseus hairy root cultures Medium optimization and elicitationPlant Cell Tissue Org. Cult.38273279CrossRefGoogle Scholar
  28. Yukimune, Y, Tabata, H, Higashi, Y, Hara, Y 1996Methyl jasmonate-induced overproduction of paclitaxel and baccatin III in Taxus cell suspension culturesNat. Biotechnol.1411291132CrossRefPubMedGoogle Scholar

Copyright information

© Springer 2006

Authors and Affiliations

  1. 1.Department of Botany, Centre of Advanced Study in Cell and Chromosome ResearchUniversity of CalcuttaCalcuttaIndia
  2. 2.Department of BotanyRKMVC CollegeRaharaIndia

Personalised recommendations