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Aluminium Chloride Enhances Colchicine Production in Root Cultures of Gloriosa superba

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Abstract

Root cultures of Gloriosa superba were treated with 5 mm methyl jasmonate and 125 μm AlCl3 which enhanced the intracellular colchicine content of the roots by 50-fold and 63-fold, respectively. Ten millimolar of CaCl2 and 1 mm CdCl2 enhanced biomass significantly (7- to 8.6-fold, respectively) while maximum release of colchicine into the medium was obtained with 10 mm CdCl2. Casein hydrolysate, yeast extract and silver nitrate had no significant effect on growth and colchicine accumulation in root cultures.

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Authors and Affiliations

  1. Department of Botany, Centre of Advanced Study in Cell and Chromosome Research, University of Calcutta, 35 Ballygunge Circular Road, 700019, Calcutta, India

    Seemanti Ghosh & Sumita Jha

  2. Department of Botany, RKMVC College, 24 Parganas (North), Rahara, West Bengal, India

    Biswajit Ghosh

Authors
  1. Seemanti Ghosh
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  2. Biswajit Ghosh
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  3. Sumita Jha
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Correspondence to Sumita Jha.

Additional information

Revisions requested 2 November 2005; Revisions received 9 January 2006

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Ghosh, S., Ghosh, B. & Jha, S. Aluminium Chloride Enhances Colchicine Production in Root Cultures of Gloriosa superba. Biotechnol Lett 28, 497–503 (2006). https://doi.org/10.1007/s10529-006-0004-9

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  • DOI: https://doi.org/10.1007/s10529-006-0004-9

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