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Rapid Affinity Purification Processes for Cyclodextrin Glycosyltransferase from Bacillus circulans

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Abstract

Two rapid and easy-to-scale-up methods for the purification of cyclodextrin glycosyltransferase (CGTase) from Bacillus circulans were developed: affinity precipitation with starch and aqueous two-phase partition. The first method, optimised by a factorial design, gave an 80% CGTase adsorption at 11% starch and 1.6% ammonium sulphate, and a 65% recovery after elution with 10 mM α-cyclodextrin. The purification factor was 17. Aqueous two-phase partition yielded a 72% CGTase recovery in a two-step procedure; CGTase was obtained in the bottom phase with a purification factor of 37.

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References

  • L Bovetto D Backer J Villette P Sicard S Bouquelet (1992) ArticleTitleCyclomaltodextrin glucanotransferase from Bacillus circulans E 192 Biotechnol. Appl. Biochem 15 48–58 Occurrence Handle1532314

    PubMed  Google Scholar 

  • S Ferrarotti A Rosso M Marechal N Krymkiewicz L Marechal (1996) ArticleTitleIsolation of two strains (S-R type) of Bacillus circulans and purification of a cyclomaltodextrin-glucanotransferase Cell. Molec. Biol 42 653–657

    Google Scholar 

  • BN Gawande AY Patkar (2001) ArticleTitlePurification and properties of a novel raw starch degrading-cyclodextrin glycosyltransferase from Klebsiella pneumoniae As−22 Enzyme Microb. Technol 28 735–743 Occurrence Handle10.1016/S0141-0229(01)00347-7 Occurrence Handle11397453

    Article  PubMed  Google Scholar 

  • A Goel S Nene (1995) ArticleTitleModifications in the phenolphtalein method for spectrophotometric estimation of beta cyclodextrin Starch 47 399–400

    Google Scholar 

  • M Kennedy D Krouse (1999) ArticleTitleStrategies for improving fermentation medium performance: A review J. Ind. Microbiol. Biotechnol 23 456–475 Occurrence Handle10.1038/sj.jim.2900755

    Article  Google Scholar 

  • KH Kim HG Lim JH Seo (1993) ArticleTitleProduction of beta-cyclodextrin from starch by cyclodextrin glycosyltransferase from alkalophilic Bacillus sp E1. Kor. J. Food Sci. Technol 25 608–610

    Google Scholar 

  • T Kometani Y Terada T Nishimura H Takii S Okada (1994) ArticleTitlePurification and characterization of cyclodextrin glucanotransferase from an alkalophilic Bacillus species and transglycosylation at alkaline pHs Biosci. Biotech. Biochem 58 517–520

    Google Scholar 

  • MR Kula (1990) ArticleTitleTrends and future of aqueous two-phase extraction Bioseparation 1 181–189 Occurrence Handle1368168

    PubMed  Google Scholar 

  • RF Martins R Hatti-Kaul (2002) ArticleTitleA new cyclodextrin glycosyltransferase from an alkaliphilic Bacillus agaradhaerens isolate: purification and characterization Enzyme Microb. Technol 30 116–124 Occurrence Handle10.1016/S0141-0229(01)00461-6

    Article  Google Scholar 

  • P Pongsawasdi M Yagisawa (1988) ArticleTitlePurification and some properties of cyclomaltodextrin glucanotransferase from Bacillus circulans Agric. Biol. Chem 52 1099–1103

    Google Scholar 

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Authors and Affiliations

  1. Departamento de Ciencias Básicas, Universidad Nacional de Luján, Rutas 5 y 7, 6700, Luján, Buenos Aires, Argentina

    A. Rosso, S. Ferrarotti & N. Krymkiewicz

  2. Cátedra de Microbiología Industrial y Biotecnología. Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956, 1113, Buenos Aires, Argentina

    M.V. Miranda, B.C. Nudel & O. Cascone

Authors
  1. A. Rosso
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  2. S. Ferrarotti
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  3. M.V. Miranda
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  4. N. Krymkiewicz
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  5. B.C. Nudel
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  6. O. Cascone
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Correspondence to O. Cascone.

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Rosso, A., Ferrarotti, S., Miranda, M. et al. Rapid Affinity Purification Processes for Cyclodextrin Glycosyltransferase from Bacillus circulans. Biotechnol Lett 27, 1171–1175 (2005). https://doi.org/10.1007/s10529-005-8654-6

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  • DOI: https://doi.org/10.1007/s10529-005-8654-6

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