Abstract
Transgenic Laminaria japonica gametophytes producing a recombinant tissue-type plasminogen activator (rtPA) protein, which is an effective third-generation thrombolytic agent for acute myocardial infarction (AMI), were cultured in an illuminated bubble column bioreactor. A maximum final dry cell weight of 1120 mg l−1 was obtained in batch culture with an initial dry cell weight of 126 mg l−1 and with aeration rate of 1.2 l air min−1 l−1 culture, nitrate at 1.5 mM and phosphate at 0.17 mM. The yield of rtPA was 56 μg g−1 dry cell wt. This is the first report regarding cultivation of a transgenic macroalga in a bioreactor.
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Revisions requested 27 January 2005 and 14 April 2005; Revisions received 6 April 2005 and 17 May 2005
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Gao, J., Zhang, Y., Wang, H. et al. Suspension culture of gametophytes of transgenic kelp in a photobioreactor. Biotechnol Lett 27, 1025–1028 (2005). https://doi.org/10.1007/s10529-005-8098-z
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DOI: https://doi.org/10.1007/s10529-005-8098-z