Skip to main content
Log in

Autohydrogenotrophic Denitrifying Microbial Community in a Glass Beads Biofilm Reactor

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

A glass bead biofilm reactor was operated using H2 as an electron donor to remove nitrate at 150 mg NO3–N l−1 to below detection level. The microbial community in the glass beads biofilm reactor was investigated by using denaturing gradient gel electrophoresis (DGGE) and phylogenetic analysis. In DGGE analysis of the biofilm, five bands were dominant and indicated the presence of eight β-proteobacteria, one γ-proteobacteria and twelve clostridia. An unculturable Hydrogenophaga sp., which is a new genus of hydrogen-oxidizing bacterium was dominant in microbial community of the biofilm reactor.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • RI Amann W Ludwig KK Schleifer (1995) ArticleTitlePhylogenetic identification and in situ detection of individual microbial cells without cultivation Microbiol. Rev 59 143–169 Occurrence Handle7535888

    PubMed  Google Scholar 

  • InstitutionalAuthorNameAPHA, AWWA, WPCF (1995) Standard Methods for the Examination of Water and Wastewater EditionNumber19 American Public Health Association, American Water Works Association and Water Pollution Control Federation New York, NY

    Google Scholar 

  • F Azam DC Smith CF Steward A Hagstrom (1993) ArticleTitleBactriea-organic matter coupling and its significance for ocean carbon cycling Microbiol. Ecol 28 167–179 Occurrence Handle10.1007/BF00166806

    Article  Google Scholar 

  • AT Brain JW Lenly JJ Alvarez (1998) ArticleTitleFe(0)-supported autotrophic denitrification Environ. Sci. Technol 32 634–639 Occurrence Handle10.1021/es9707769

    Article  Google Scholar 

  • TC Driscoll JJ Bisogni (1978) ArticleTitleThe use of sulfur and sulfide in packed bed reactors for autotrophic denitrification J. Water Poll. Control Fedn 3 569–577

    Google Scholar 

  • DC Kross GR Hallberg DR Bruner K Cherryholmes JK Johnson (1993) ArticleTitleThe nitrate contamination of private well water in Iowa Am. J. Public Health 83 270–272 Occurrence Handle8427340

    PubMed  Google Scholar 

  • LA Lawrence PL McCarty (1970) ArticleTitleUnified basis for biological treatment design and operation J. San. Eng. (American Society of Civil Engineers) 6 IssueIDSA3 757

    Google Scholar 

  • G Muyzer EC Waal Particlede AG Uitterlinden (1993) ArticleTitleProfiling of complex microbial population by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA Appl. Environ. Microbiol 59 659–700

    Google Scholar 

  • HI Park JS Kim DK Kim DW Pak (2004) ArticleTitleAutohydrogenotrophic denitrification of high nitrate concentration in a glass beads biofilm reactor J. Kor. Soc. Water Quality 20 236–240

    Google Scholar 

  • BE Rittmann V Snoeyink (1984) ArticleTitleAchieving biologically stable drinking water J. Am. Water Works Assoc 76 106–114

    Google Scholar 

  • V Urbain R Benoid J Manem (1996) ArticleTitleMembrane bioreactor: a new treatment tool J. Am. Water Works Assoc 88 75–86

    Google Scholar 

  • A Willems J Busse M Goor B Pot (1989) ArticleTitleHydrogenophaga, a new genus of hydrogen-oxidizing bacteria that includes Hydrogenophaga flava comb. nov. (formerly Pseudomonas flava), Hydrogenophaga palleronii (formerly Pseudomonas palleronii), Hydrogenophaga pseudoflava (formerly Pseudomonas pseudoflava and Pseudomonas carboxydoflava) and Hydrogenophaga taeniospiralis (formerly Pseudomonas taeniospiralis) Int. J. Syst. Bacteriol 39 319–333

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Daewon Pak.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Park, H.I., Choi, YJ. & Pak, D. Autohydrogenotrophic Denitrifying Microbial Community in a Glass Beads Biofilm Reactor. Biotechnol Lett 27, 949–953 (2005). https://doi.org/10.1007/s10529-005-7654-x

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-005-7654-x

Keywords

Navigation