Abstract
Expression of the desulfurization genes (dsz) in Mycobacterium sp. G3 is repressed by sulfate, which is the product of biodesulfurization. An expression clone, pSMTABC, was constructed by placing the dsz genes downstream of the hsp60 promoter and the constructed plasmid was electroporated into G3. The recombinant strain G3-1 desulfurized dibenzothiophene in the presence of 0.5 mM sulfate while the Dsz phenotype was completely repressed in the wild-type strain. However, there was no significant increase in the amount of desulfurization enzymes in G3-1. In addition, G3 had superior separation of diesel oil–water separation activity compared to E. coli, which is superior to desulfurizing rhodococci.
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References
AP Borole EN Kaufman MJ Grossman V Minak-Bernero R Bare MK Lee (2002) ArticleTitleComparison of the emulsion characteristics of Rhodococcus erythropolis and Escherichia coli SOXC-5 cells expressing biodesulfurization genes Biotechnol. Prog 18 88–93 Occurrence Handle10.1021/bp010134w Occurrence Handle11822905
MS Gallardo A Ferrandez VD Lorenzo JL Gatcia E Diaz (1997) ArticleTitleDesigning recombinant Pseudomonas strains to enhance biodesulfurization J. Bacteriol 179 7156–7160 Occurrence Handle9371464
E Golanska A Brozostek P Kiatpapan J Dziadek (1998) ArticleTitleCharacterisation of a new host-vector system for fast-growing mycobacteria Acta Microbiol. Pol 47 335–343 Occurrence Handle10333556
KA Gray OS Pogrebinsky GT Mrachko L Xi DJ Monticello CH Squires (1996) ArticleTitleMolecular mechanisms of biocatalytic desulfurization of fossil fuels Nature Biotechnol 14 1705–1709 Occurrence Handle10.1038/nbt1296-1705
K Hirasawa Y Ishii M Kobayashi K Koizumi K Maruhashi (2001) ArticleTitleImprovement of desulfurization activity in Rhodococcus erythropolis KA2–5-1 by genetic engineering Biosci. Biotechnol. Biochem 65 239–246 Occurrence Handle10.1271/bbb.65.239 Occurrence Handle11302154
M Kobayashi T Onaka Y Ishii J Konishi M Takaki H Okada Y Ohta K Koizumi M Suzuki (2000) ArticleTitleDesulfurization of alkylated forms of both dibenzothiophene and benzothiophene by a single bacterial strain FEMS Microbiol. Lett 187 123–126 Occurrence Handle10.1016/S0378-1097(00)00187-7 Occurrence Handle10856644
MZ Li CH Squires DJ Monticello JD Childs (1996) ArticleTitleGenetic analysis of the dsz promoter and associated regions of Rhodococcus erythropolis IGTS8 J. Bacteriol 178 6409–6418 Occurrence Handle8932295
T Matsui T Onaka Y Tanaka T Tezuka M Suzuki R Kurane (2000) ArticleTitleAlkylated benzothiophene desulfurization by Rhodococcus sp. strain T09 Biosci. Biotechnol. Biochem 64 596–599 Occurrence Handle10.1271/bbb.64.596 Occurrence Handle10803960
DJ Monticello (2000) ArticleTitleBiodesulfurization and the upgrading of petroleum distillates Curr. Opin. Biotech 11 540–546 Occurrence Handle10.1016/S0958-1669(00)00154-3 Occurrence Handle11102787
S Nekodzuka T Nakajima-Kambe N Nomura J Lu T Nakahara (1997) ArticleTitleSpecific desulfurization of dibenzothiophene by Mycobacterium sp. strain G3 Biocatal. Biotransfor 15 17–27
H Okada N Nomura T Nakahara K Maruhashi (2001) ArticleTitleCultivation of a desulfurizing bacterium, Mycobacterium strain G3 Biotechnol. Lett 23 2047–2050 Occurrence Handle10.1023/A:1013747931881
H Okada N Nomura T Nakahara K Maruhashi (2002) ArticleTitleAnalysis of substrate specificity of the desulfurizing bacterium Mycobacterium sp G3 J. Biosci. Bioeng 93 228–233 Occurrence Handle10.1263/jbb.93.228
H Okada N Nomura T Nakahara K Saitoh H Uchiyama K Maruhashi (2003) ArticleTitleAnalyses of microbial desulfurization reaction of alkylated dibenzothiophenes dissolved in oil phase Biotechnol. Bioeng 83 489–497 Occurrence Handle10.1002/bit.10694 Occurrence Handle12800143
T Onaka K Okumura M Suzuki (1997) ArticleTitleAnalysis of biodesulfurization products using solid-phase extraction J. Chromatogr. Sci 35 417–422
JR van der Ploeg E Eichhorn T Leisinger (2001) ArticleTitleSulfonate-sulfur metabolism and its regulation in Eschericha coli Arch. Microbiol 176 1–8 Occurrence Handle10.1007/s002030100298 Occurrence Handle11479697
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Takada, M., Nomura, N., Okada, H. et al. De-repression and comparison of oil–water separation activity of the dibenzothiophene desulfurizing bacterium, Mycobacterium sp. G3. Biotechnol Lett 27, 871–874 (2005). https://doi.org/10.1007/s10529-005-6721-7
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DOI: https://doi.org/10.1007/s10529-005-6721-7