Abstract
Transgenic research often suffers from the lack of strong tissue specific promoters and the lack of suitable antibodies for transgene detection. We have now constructed a novel prostate specific 2-in-1 Tetracycline-off (Tet-off), bicistronic expression vector, designated PbTetOIE, for transgenic research. The vector allows potent induction as well as inducible suppression of transgene expression in the prostate epithelial cells, and also allows the detection of transgene expression at the cellular level through the detection of the internal enhanced green fluorescent protein (EGFP) downstream of the transgene.
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DE Brookes D Zandvliet F Watt PJ Russell PL Molloy (1998) ArticleTitleRelative activity and specificity of promoters from prostate-expressed genes Prostate 35 18–26 Occurrence Handle10.1002/(SICI)1097-0045(19980401)35:1<18::AID-PROS3>3.0.CO;2-D Occurrence Handle9537595
DC Danila X Zhang Y Zhou JN Haidar A Klibanski (2002) ArticleTitleOverexpression of wild-type activin receptor alk4–1 restores activin antiproliferative effects in human pituitary tumor cells J. Clin. Endocrinol. Metab 87 4741–4746 Occurrence Handle10.1210/jc.2002-020527 Occurrence Handle12364468
PA Furth L St Onge H Boger P Gruss M Gossen A Kistner H Bujard L Hennighausen (1994) ArticleTitleTemporal control of gene expression in transgenic mice by a tetracycline-responsive promoter Proc. Natl. Acad. Sci U.S.A 91 9302–9306 Occurrence Handle7937760
L Ge P Rudolph (1997) ArticleTitleSimultaneous introduction of multiple mutations using overlap extension PCR Biotechniques 22 28–30 Occurrence Handle8994639
M Gossen AL Bonin H Bujard (1993) ArticleTitleControl of gene activity in higher eukaryotic cells by prokaryotic regulatory elements Trends. Biochem. Sci 18 471–475 Occurrence Handle10.1016/0968-0004(93)90009-C Occurrence Handle8108860
M Gossen S Freundlieb G Bender G Muller W Hillen H Bujard (1995) ArticleTitleTranscriptional activation by tetracyclines in mammalian cells Science 268 1766–1769 Occurrence Handle7792603
NM Greenberg FJ DeMayo PC Sheppard R Barrios R Lebovitz M Finegold R Angelopoulou JG Dodd ML Duckworth JM Rosen (1994) ArticleTitleThe rat probasin gene promoter directs hormonally and developmentally regulated expression of a heterologous gene specifically to the prostate in transgenic mice Mol. Endocrinol 8 230–239 Occurrence Handle10.1210/me.8.2.230 Occurrence Handle8170479
M Hennecke M Kwissa K Metzger A Oumard A Kroger R Schirmbeck J Reimann H Hauser (2001) ArticleTitleComposition and arrangement of genes define the strength of IRES-driven translation in bicistronic mRNAs Nucleic. Acids. Res 29 3327–3334 Occurrence Handle10.1093/nar/29.16.3327 Occurrence Handle11504870
RM Horton ZL Cai SN Ho LR Pease (1990) ArticleTitleGene splicing by overlap extension: tailor-made genes using the polymerase chain reaction Biotechniques 8 528–535 Occurrence Handle2357375
H Lottmann J Vanselow B Hessabi R Walther (2001) ArticleTitleThe Tet-On system in transgenic mice: inhibition of the mouse pdx-1 gene activity by antisense RNA expression in pancreatic beta-cells J. Mol. Med 79 321–328 Occurrence Handle10.1007/s001090100229 Occurrence Handle11485027
S Rubinchik D Wang H Yu F Fan M Luo JS Norris JY Dong (2001) ArticleTitleA complex adenovirus vector that delivers FASL–GFP with combined prostate-specific and tetracycline-regulated expression Mol. Ther 4 416–426 Occurrence Handle10.1006/mthe.2001.0478 Occurrence Handle11708878
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Young, L., Dong, Q. Development of a murine prostatespecific 2-in-1 inducible bicistronic expression vector. Biotechnol Lett 27, 823–828 (2005). https://doi.org/10.1007/s10529-005-6134-7
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DOI: https://doi.org/10.1007/s10529-005-6134-7