Abstract
Cell adhesion to extracellular matrices, including fibronectin, results in clustering of integrins in focal adhesions. To promote the clustering of fibronectin and thus enhance its activity at the sites of focal adhesion formation, we have engineered a fusion protein containing recombinant fibronectin fragment (hFN) connected to the tetramerization helix domain of lac repressor for oligomeric assembly. Purified Lac-hFN fusion protein exhibited significant increase of cell adhesion and proliferation of GF cells compared with hFN alone (p < 0.05).
Similar content being viewed by others
References
S Aota M Nomizu KM Yamada (1994) ArticleTitleThe short amino acid sequence Pro-His-Ser-Arg-Asn in human fibronectin enhances cell-adhesive function J. Biol. Chem 269 24756–24761 Occurrence Handle7929152
CA Buck AF Horwitz (1987) ArticleTitleCell surface receptors for extracellular matrix molecules Annu. Rev. Cell Biol 3 179–205 Occurrence Handle10.1146/annurev.cb.03.110187.001143 Occurrence Handle2825736
K Burridge M Chrzanowska-Wodnicka (1996) ArticleTitleFocal adhesions, contractility, and signaling Annu. Rev. Cell Dev. Biol 12 463–518 Occurrence Handle10.1146/annurev.cellbio.12.1.463 Occurrence Handle8970735
D Choquet DP Felsenfeld MP Sheetz (1997) ArticleTitleExtracellular matrix rigidity causes strengthening of integrin-cytoskeleton linkages Cell 88 39–48 Occurrence Handle10.1016/S0092-8674(00)81856-5 Occurrence Handle9019403
PH Choung BM Seo CP Chung KM Yamada JH Jang (2002) ArticleTitleSynergistic activity of fibronectin and fibroblast growth factor receptors on neuronal adhesion and neurite extension through extracellular signal-regulated kinase pathway Biochem. Biophys. Res. Commun 295 898–902 Occurrence Handle10.1016/S0006-291X(02)00774-X Occurrence Handle12127979
P Geer Particlevan der T Hunter RA Lindberg (1994) ArticleTitleReceptor protein-tyrosine kinases and their signal transduction pathways Annu. Rev. Cell Biol 10 251–337 Occurrence Handle10.1146/annurev.cb.10.110194.001343 Occurrence Handle7888178
MJ Humphries (1996) ArticleTitleIntegrin activation: the link between ligand binding and signal transduction Cure Opin. Cell Biol 8 632–640 Occurrence Handle10.1016/S0955-0674(96)80104-9
RO Hynes (2002) ArticleTitleIntegrins: bidirectional, allosteric signaling machines Cell 110 673–687 Occurrence Handle10.1016/S0092-8674(02)00971-6 Occurrence Handle12297042
JH Jang Y Ku CP Chung SJ Heo (2002) ArticleTitleEnhanced fibronectin-mediated cell adhesion of human osteoblast by fibroblast groth factor, FGF-2 Biotechnol. Lett 24 1659–1663 Occurrence Handle10.1023/A:1020617904251
JH Jang KH Shin YJ Park RJ Lee WL McKeehan JG Park (2000) ArticleTitleNovel transcripts of fibroblast growth factor receptor 3 reveal aberrant splicing and activation of cryptic splice sequences in colorectal cancer Cancer Res 60 4049–4052 Occurrence Handle10945607
N Lehming J Sartorius S Oehler B Wilcken-Bergmann Particle von B Muller-Hill (1988) ArticleTitleRecognition helices of lac and lambda repressor are oriented in opposite directions and recognize similar DNA sequences Proc. Natl. Acad. Sci. USA 85 7947–7951 Occurrence Handle3186699
M Lewis G Chang NC Horton MA Kercher HC Pace MA Schumacher RG Brennan P Lu (1996) ArticleTitleCrystal structure of the lactose operon repressor and its complexes with DNA and inducer Science 271 1247–1254 Occurrence Handle8638105
S Miyamoto H Teramoto JS Gutkind KM Yamada (1996) ArticleTitleIntegrins can collaborate with growth factors for phosphorylation of receptor tyrosine kinases and MAP kinase activation: roles of integrin aggregation and occupancy of receptors J. Cell Biol 135 1633–1642 Occurrence Handle10.1083/jcb.135.6.1633 Occurrence Handle8978828
BC Peterson RH Rownd (1983) ArticleTitleHomologous sequences other than insertion elements can serve as recombination sites in plasmid drug resistance gene amplification J. Bacteriol 156 177–185 Occurrence Handle6311796
WS Reznikoff JH Miller JG Scaife JR Beckwith (1969) ArticleTitleA mechanism for repressor action J. Mol. Biol 43 201–213 Occurrence Handle10.1016/0022-2836(69)90089-8 Occurrence Handle4897791
AD Riggs S Bourgeois RF Newby M Cohn (1968) ArticleTitleDNA binding of the lac repressor J. Mol. Biol 34 365–368 Occurrence Handle10.1016/0022-2836(68)90261-1 Occurrence Handle4938552
E Ruoslahti (1988) ArticleTitleFibronectin and its receptors Annu Rev Biochem 57 375–413 Occurrence Handle10.1146/annurev.bi.57.070188.002111 Occurrence Handle2972252
E Ruoslahti MD Pierschbacher (1987) ArticleTitleNew perspectives in cell adhesion: RGD and integrins Science 238 491–497 Occurrence Handle2821619
SK Sastry K Burridge (2000) ArticleTitleFocal adhesions: a nexus for intracellular signaling and cytoskeletal dynamics Exp. Cell Res 261 25–36 Occurrence Handle10.1006/excr.2000.5043 Occurrence Handle11082272
DD Schlaepfer T Hunter (1998) ArticleTitleIntegrin signalling and tyrosine phosphorylation: just the FAKs? Trends Cell Biol 8 151–157 Occurrence Handle10.1016/S0962-8924(97)01172-0 Occurrence Handle9695829
A Woods JR Couchman (1992) ArticleTitleProtein kinase C involvement in focal adhesion formation Cell Sci 101 IssueIDPt 2 277–290
A Woods JB McCarthy LT Furcht JR Couchman (1993) ArticleTitleA synthetic peptide from the COOH-terminal heparin-binding domain of fibronectin promotes focal adhesion formation Mol. Biol. Cell 4 605–613 Occurrence Handle8374170
X Zhu RK Assoian (1995) ArticleTitleIntegrin-dependent activation of MAP kinase: a link to shape-dependent cell proliferation Mol. Biol. Cell 6 273–282 Occurrence Handle7612963
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Chung, CP., Jang, JH. Design and expression of oligomeric fibronectin fusion protein: a strategy for enhancing cell adhesion activity. Biotechnol Lett 27, 811–816 (2005). https://doi.org/10.1007/s10529-005-6101-3
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10529-005-6101-3