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Differential Gene Expression of Human CD34+ Hematopoietic Stem and Progenitor Cells Before and After Culture

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Abstract

Ex vivo expanded CD34+ hematopoietic stem and progenitor cells (HSPCs) have compromised homing and engraftment capacities. To investigate underlying mechanisms for functional changes of expanded HSPCs, we compared gene expression profiling of cultured and fresh CD34+ cells derived from cord blood using SMART-PCR and cDNA array: 20 genes were up-regulated while 25 genes were down-regulated in cultured CD34+ HSPCs. These differentially expressed genes are involved primarily in proliferation, differentiation, apoptosis, and homing.

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Authors and Affiliations

  1. The State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 200237, Shanghai, China

    Qunliang Li, Haibo Cai, Qiwei Liu & Wen-Song Tan

Authors
  1. Qunliang Li
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  2. Haibo Cai
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  3. Qiwei Liu
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  4. Wen-Song Tan
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Corresponding author

Correspondence to Wen-Song Tan.

Additional information

Revisions requested 27 September 2005; Revisions received 14 December 2005

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Li, Q., Cai, H., Liu, Q. et al. Differential Gene Expression of Human CD34+ Hematopoietic Stem and Progenitor Cells Before and After Culture. Biotechnol Lett 28, 389–394 (2006). https://doi.org/10.1007/s10529-005-6064-4

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  • DOI: https://doi.org/10.1007/s10529-005-6064-4

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