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Presence of Unintended Agrobacterium tumefaciens Cloning Vector Sequences in Genetically Modified Plants

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Abstract

Agrobacterium transformation was used in the production of genetically modified plants from oilseed rape (Brassica napus) and tobacco (Nicotiana tabacum). After inoculation stop with the antibiotic timentin, a subsequent one-week treatment eliminated the vector bacterium from the oilseed rape plate explant cultures. From the tobacco, however, we recorded vector-derived signals one week after potting the regenerants in the greenhouse and still 10 weeks later. Genetically modified plants produced through Agrobacterium-transformation therefore cannot be guaranteed to be completely free of unintended vector sequences after antibiotic treatment.

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References

  • M Bevan (1984) ArticleTitleBinary Agrobacterium vectors for plant transformation Nucleic Acids Res. 12 8711–8717 Occurrence Handle1:CAS:528:DyaL2MXktV2gug%3D%3D Occurrence Handle6095209

    CAS  PubMed  Google Scholar 

  • SB Gelvin (2003) ArticleTitleAgrobacterium-mediated plant transformation: the biology behind the “gene-Jockeying” tool Microbiol. Mol. Biol. Rev. 67 16–37 Occurrence Handle1:CAS:528:DC%2BD3sXis1yjsLw%3D Occurrence Handle12626681

    CAS  PubMed  Google Scholar 

  • JI Ghezzi TR Steck (1999) ArticleTitleInduction of the viable but non-culturable condition in Xanthomonas campestris pv. campestris in liquid microcosms and sterile soil FEMS Microbiol. Ecol. 30 203–208 Occurrence Handle1:CAS:528:DyaK1MXmsFahsrk%3D Occurrence Handle10525176

    CAS  PubMed  Google Scholar 

  • B Hanson D Engler Y Moy B Newman E Ralston N Gutterson (1999) ArticleTitleA simple method to enrich an Agrobacterium-transformed population for plants containing only T-DNA sequences Plant J. 19 727–734 Occurrence Handle10.1046/j.1365-313x.1999.00564.x Occurrence Handle1:CAS:528:DyaK1MXntFOnsbk%3D Occurrence Handle10571858

    Article  CAS  PubMed  Google Scholar 

  • A Hoekema PR Hirsch PJJ Hooykaas RA Schilperoort (1983) ArticleTitleA binary plant vector strategy based on separation of vir and T-region of the A. tumefaciens Ti-plasmid Nature 303 179–180 Occurrence Handle10.1038/303179a0 Occurrence Handle1:CAS:528:DyaL3sXktVWhsrY%3D

    Article  CAS  Google Scholar 

  • ME Kononov B Bassuner SB Gelvin (1997) ArticleTitleIntegration of T-DNA binary ‘backbone’ sequences into the tobacco genome: evidence for multiple complex patterns of integration Plant J. 11 945–957 Occurrence Handle10.1046/j.1365-313X.1997.11050945.x Occurrence Handle1:CAS:528:DyaK2sXkt1els7c%3D Occurrence Handle9193068

    Article  CAS  PubMed  Google Scholar 

  • C Leifert AC Cassells (2001) ArticleTitleMicrobial hazards in plant tissue and cell cultures In Vitro Cell. Dev. Biol. Plant 37 133–138

    Google Scholar 

  • R Martí J Cubero A Daza J Piquer CI Salcedo C Morente MM López (1999) ArticleTitleEvidence of migration and endophytic presence of Agrobacterium tumefaciens in rose plants Eur. J. Plant Pathol. 105 39–50 Occurrence Handle10.1023/A:1008660500107

    Article  Google Scholar 

  • M Seyring (1999) ArticleTitleEffect of antibiotics on elimination of Agrobacterium tumefaciens and the growth of shoot cultures of Argyranthemum frutescens in vitro and in vivo Gartenbauwissenschaft 64 177–182 Occurrence Handle1:CAS:528:DC%2BD3cXhvVOmsLg%3D

    CAS  Google Scholar 

  • MA Surette AV Sturz RR Lada J Nowak (2003) ArticleTitleBacterial endophytes in processing carrots (Daucus carota L. var. sativus): their localization, population density, biodiversity and their effects on plant growth Plant Soil 253 381–390 Occurrence Handle10.1023/A:1024835208421 Occurrence Handle1:CAS:528:DC%2BD3sXlsFKqsrs%3D

    Article  CAS  Google Scholar 

  • S Teyssier-Cuvelle C Mougel X Nesme (1999) ArticleTitleDirect conjugal transfers of Ti-plasmid to soil microflora Mol. Ecol. 8 1273–1284 Occurrence Handle10.1046/j.1365-294X.1999.00689.x Occurrence Handle1:CAS:528:DyaK1MXlvVOitb4%3D Occurrence Handle10447868

    Article  CAS  PubMed  Google Scholar 

  • SA Weller SA Simpkins DE Stead A Kurdziel H Hird RJ Weekes (2002) ArticleTitleIdentification of Agrobacterium spp. present within Brassica napus seed by Taqman PCR – Implications for GM screening procedures Arch. Microbiol. 178 338–343 Occurrence Handle10.1007/s00203-002-0461-z Occurrence Handle1:CAS:528:DC%2BD38XnslaqtrY%3D Occurrence Handle12375101

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Research Department, Finnish Environment Institute, Hakuninmaantie 6, FI-00430, Helsinki, Finland

    Katarina Björklöf & Kirsten S. Jørgensen

  2. UniCrop Ltd, Helsinki Business and Science Park, Viikinkaari 4, FI-00790, Helsinki, Finland

    Michael Färdig

Authors
  1. Katarina Björklöf
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  2. Michael Färdig
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  3. Kirsten S. Jørgensen
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Correspondence to Katarina Björklöf.

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Björklöf, K., Färdig, M. & Jørgensen, K.S. Presence of Unintended Agrobacterium tumefaciens Cloning Vector Sequences in Genetically Modified Plants. Biotechnol Lett 28, 341–349 (2006). https://doi.org/10.1007/s10529-005-5933-1

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  • DOI: https://doi.org/10.1007/s10529-005-5933-1

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