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Kinetic Analysis of the ex vivo Expansion of Human Hematopoietic Stem/Progenitor Cells

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Abstract

The total cell expansion of human umbilical cord blood (CB) and adult bone marrow (BM) CD34+-enriched cells cultured in supplemented serum-free media, either over irradiated human feeder layers or in stroma-free systems, were characterized by a simple kinetic model using only two parameters: the specific cell expansion rate, μ, and the death rate constant, k k . Both CB and BM cells can expand at approximately the same rate (0.21 day−1) in this culture system however, cell death depends on the presence of stroma and the environment in which the cells are cultured.

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Authors and Affiliations

  1. Centro de Engenharia Biológica e Química, Instituto Superior Técnico, Av. Rovisco Pais, 1049-001, Lisboa, Portugal

    Raquel Gonçalves, Cláudia Lobato da Silva, Bruno S. Ferreira, Maria Amélia N. D. A. Lemos, Francisco Lemos, Graça Almeida-Porada & Joaquim M. S. Cabral

  2. Department of Animal Biotechnology, University of Nevada, Reno, 89557, USA

    Raquel Gonçalves & Cláudia Lobato da Silva

Authors
  1. Raquel Gonçalves
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  2. Cláudia Lobato da Silva
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  3. Bruno S. Ferreira
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  4. Maria Amélia N. D. A. Lemos
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  5. Francisco Lemos
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  6. Graça Almeida-Porada
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  7. Joaquim M. S. Cabral
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Corresponding author

Correspondence to Joaquim M. S. Cabral.

Additional information

Raquel Gonçalves and Cláudia Lobato da Silva - These authors contributed equally to this work.

Revisions requested 3 November 2005; Revisions received 5 December 2005

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Gonçalves, R., Silva, C.L.d., Ferreira, B.S. et al. Kinetic Analysis of the ex vivo Expansion of Human Hematopoietic Stem/Progenitor Cells. Biotechnol Lett 28, 335–340 (2006). https://doi.org/10.1007/s10529-005-5932-2

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  • DOI: https://doi.org/10.1007/s10529-005-5932-2

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