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Mist trickling bioreactor for Centaurium erythraea Rafn growth of shoots and production of secoiridoids

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Abstract

Shoots of Centaurium erythraea Rafn were cultivated in 5 l mist trickling bioreactor for 21 and 28 days increasing their dry weight from 0.54 g to 13.7 g and 18.3 g, respectively. About 6880 shoots from 223 initial shoot-tips in 21-day bioreactor producing cycle were produced. The shoots could be successfully rooted and transferred to soil. Secoiridoid accumulation (expressed as a sum of gentiopicroside, sweroside and swertiamarin) in shoots after 21 days of culture reached about 303 mg l−1.

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Author notes

  1. Ewelina Piatczak

    Present address: Department of Biology and Pharmaceutical Botany, Medical University, Kochanowskiego Str. 10, 51-601, Wroclaw, Poland

Authors and Affiliations

  1. Department of Pharmaceutical Biology and Biotechnology, Medical University, Muszynskiego Str. 1, 90–151, Lodz, Poland

    Aleksander Chmiel & Halina Wysokinska

Authors
  1. Ewelina Piatczak
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  2. Aleksander Chmiel
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  3. Halina Wysokinska
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Correspondence to Ewelina Piatczak.

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Piatczak, E., Chmiel, A. & Wysokinska, H. Mist trickling bioreactor for Centaurium erythraea Rafn growth of shoots and production of secoiridoids. Biotechnol Lett 27, 721–724 (2005). https://doi.org/10.1007/s10529-005-5189-9

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  • DOI: https://doi.org/10.1007/s10529-005-5189-9

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