Abstract
The nutrition conditions needed to redirect the carbon flux in Torulopsis glabrata, a pyruvate hyper-production yeast, from pyruvate to α-ketoglutaric acid (KG) were investigated in a stirred fermentor. A minor amount of KG (1.3 gl−1) was produced when NaOH was used to control the pH, while 12 g KG l−1 was produced when CaCO3 was used instead. When thiamine and biotin were included in the medium, 13 g KG l−1 and 68 g pyruvate l−1 were produced after 48 h when glucose was nearly consumed (approximately 5 gl−1). With fermentation continuing for a further 16 h, the concentration of pyruvate decreased to 31 gl−1, and KG increased to 30 gl−1. KG thus accumulated at the expense of pyruvate consumption.
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Received 2 June 2005; Revisions requested 30 June 2005 and 1 September 2005; Revisions received 1 September 2005 and 28 October 2005; Accepted 28 October 2005
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Huang, H.J., Liu, L.M., Li, Y. et al. Redirecting Carbon Flux in Torulopsis glabrata from Pyruvate to α-Ketoglutaric Acid by Changing Metabolic Co-factors. Biotechnol Lett 28, 95–98 (2006). https://doi.org/10.1007/s10529-005-4953-1
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DOI: https://doi.org/10.1007/s10529-005-4953-1