Abstract
A sensitive method for quantification of S-adenosyl methionine (SAM) in microbial cell extracts was developed and applied to Corynebacterium glutamicum. The method is based on SAM being completely hydrolyzed into 18O-homoserine when extracted in boiling H 182 O and thus can be clearly distinguished by GC-MS analysis from naturally labeled homoserine present in the cell extract. Additional quantification of the total homoserine pool, representing both SAM and homoserine, via HPLC allows separate determination of both metabolites.
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Received 23 August 2005; Revisions requested 30 August 2005; Revisions received 26 September 2005; Accepted 31 October 2005
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Krömer, J.O., Heinzle, E. & Wittmann, C. Quantification of S-adenosyl Methionine in Microbial Cell Extracts. Biotechnol Lett 28, 69–71 (2006). https://doi.org/10.1007/s10529-005-4947-z
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DOI: https://doi.org/10.1007/s10529-005-4947-z