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Production of eleutherosides in in vitro regenerated embryos and plantlets of Eleutherococcus chiisanensis

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Abstract

High frequency somatic embryogenesis of Eleutheorcoccus chiisanensis was achieved through suspension culture of embryogenic cells in hormone-free Murashige and Skoog liquid medium supplemented with 30 g sucrose l−1. Cotyledonary somatic embryos were germinated and converted into plantlets using 20 μM gibberellic acid which were then grown in a 10 l airlift bioreactor. HPLC analysis revealed the accumulation of eleutheroside B, E and E1 in the embryos and plantlets. Thus mass production of embryos and plantlets of E. chiisanensis can be achieved in liquid cultures and the biomass produced may become an alternative source of eleutherosides.

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Authors and Affiliations

  1. Biotechnology Division, Korea Forest Research Institute, Suwon, 441-350, Korea

    J.H. Jeong & H.K. Moon

  2. Department of Medicinal Resources and Horticulture, Namdo Provincial College, Jeonnam, Changhung, 529-850, Korea

    J.H. Jeong

  3. Division of Forest Resources, College of Forest Sciences, Kangwon National University, Chuchon, 200-701, Korea

    S.J. Jung & Y.E. Choi

  4. Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheongju, 361-763, Korea

    H.N. Murthy, K.W. Yu & K.Y. Paek

  5. Department of Botany, Karnatak University, Dharwad, 58003, India

    H.N. Murthy

Authors
  1. J.H. Jeong
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  2. S.J. Jung
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  3. H.N. Murthy
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  4. K.W. Yu
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  5. K.Y. Paek
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  6. H.K. Moon
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  7. Y.E. Choi
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Corresponding author

Correspondence to H.N. Murthy.

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Jeong, J., Jung, S., Murthy, H. et al. Production of eleutherosides in in vitro regenerated embryos and plantlets of Eleutherococcus chiisanensis. Biotechnol Lett 27, 701–704 (2005). https://doi.org/10.1007/s10529-005-4693-2

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  • DOI: https://doi.org/10.1007/s10529-005-4693-2

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