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Immobilization of Cryopreserved Primary Rat Hepatocytes for the Development of a Bioartificial Liver System

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Abstract

Primary rat hepatocytes were cryopreserved in hormonally-defined medium (HDM) containing 40% (v/v) fetal bovine serum (FBS) and 10% (v/v) dimethyl sulfoxide (DMSO) in liquid N2 for 6 months. After thawing, the cells were immobilized using 2% (w/v) alginate and 0.5% (w/v) chitosan solutions. The capacities of ammonia removal and urea synthesis of the immobilized-thawed hepatocytes were similar to those of immobilized hepatocytes without cryopreservation. This result shows that immobilized hepatocytes after cryopreservation are useful for the development of a bioartificial liver system.

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Authors and Affiliations

  1. Department of Biotechnology and Bioengineering, Pukyong National University, 608-737, Busan, Korea

    Jeong Hwa Son, Yu-Mi Ha, Yun-Im Kim, Ki Myong Kim & Sung-Koo Kim

  2. Department of Chemical Engineering, Dongguk University, 100-715, Seoul, Korea

    Jung-Keug Park

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  1. Jeong Hwa Son
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  2. Yu-Mi Ha
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  3. Yun-Im Kim
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  4. Ki Myong Kim
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  5. Jung-Keug Park
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  6. Sung-Koo Kim
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Correspondence to Sung-Koo Kim.

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Son, J.H., Ha, YM., Kim, YI. et al. Immobilization of Cryopreserved Primary Rat Hepatocytes for the Development of a Bioartificial Liver System. Biotechnol Lett 28, 51–54 (2006). https://doi.org/10.1007/s10529-005-4687-0

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  • DOI: https://doi.org/10.1007/s10529-005-4687-0

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