Abstract
Sodium citrate, added to culture medium at 2 g l−1, increased inosine production by B. subtilis by 18% to 16 g l−1. The phosphoenolpyruvate (PEP) pool was increased by 2.3-fold, while the pyruvate and acetate pools were decreased by 78% and 57%, respectively. Pyruvate kinase might thus be a regulatory site for inosine synthesis.
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References
AM Alves GJ Euverink MJ Bibb L Dijkhuizen (1997) ArticleTitleIdentification of ATP-dependent phosphofructokinase as a regulatory step in the glycolytic pathway of the Actinomycete streptomyces coelicolor A3 (2) Appl. Environ. Microbiol 63 956–961 Occurrence Handle9055413
A Boiteux M Markus T Plesser B Hess M Malcovati (1983) ArticleTitleInteraction of pyruvate kinase from Escherichia coli with fructose 1,6-biphosphate and calcium ions Biochem. J 211 631–640 Occurrence Handle6349612
SH Fisher B Mangasanik (1984) ArticleTitleSynthesis of oxaloacetate in Bacillus subtilis mutants lacking the 2-ketoglutarate dehydrogenase enzymatic complex J. Bacteriol 158 55–62 Occurrence Handle6425269
P Fortnagel E Freese (1968) ArticleTitleAnalysis of sporulation mutants II. Mutants blocked in the citric acid cycle J. Bacteriol 95 1434–1438
A Goel J Lee MM Domach MM Ataai (1995) ArticleTitleSuppressed acid formation by cofeeding glucose and citrate in Bacilluscultures: emergence of pyruvate kinase as a potential metabolic engineering site Biotechnol. Prog 11 380–386 Occurrence Handle10.1021/bp00034a003 Occurrence Handle7654309
A Goel J Lee MM Domach MM Ataai (1999) ArticleTitleMetabolic fluxes, pool and enzyme measurements suggest a tighter coupling of energetics and biosynthetic reactions associated with reduced pyruvate kinase flux Biotechnol. Bioeng 64 129–134 Occurrence Handle10.1002/(SICI)1097-0290(19990720)64:2<129::AID-BIT1>3.0.CO;2-I Occurrence Handle10397848
R Horne W Anderson R Nordlie (1970) ArticleTitleGlucose dehydrogenase activity of yeast glucose-6-phosphate dehydrogenase. Inhibition by adenosine 5′-triphosphate and other nucleoside 5′-triphosphates and diphosphate Biochemistry 9 610–615 Occurrence Handle10.1021/bi00805a023 Occurrence Handle4391971
M Malcovati G Valentini (1982) ArticleTitleAMP and fructose 6-phophate activated pyruvate kinase from E. coli Meth. Enzymol 90 170–179 Occurrence Handle6759852
T Palme K Tiisma A Kahru R Vilu (1990) ArticleTitleGlucose-limited fed-batch cultivation of Escherichia coli with computer-con-trolled fixed growth rate Biotechnol. Bioeng 35 312–319 Occurrence Handle10.1002/bit.260350313
E Postma WA Scheffers JP Dijken Particlevan (1988) ArticleTitleAdaptation of the kinetics of glucose transport to environmental conditions in the yeast Candida utilis CBS 621 continuous-culture study J. Gen. Microbiol 134 1109–1116
PW Postma JW Lengeler GR Jacobson (1993) ArticleTitlePhosphoenolpyruvate: carbohydrate phosphotransferase systems of bacteria Microbiol. Rev 57 543–594 Occurrence Handle8246840
K Willecke AB Pardee (1971) ArticleTitleInducible transport of citrate in Gram-positive bacteria, B.subtilis J. Biol. Chem 246 1032–1040 Occurrence Handle4993437
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Chen, S., Chu, J., Zhuang, Y. et al. Enhancement of inosine production by Bacillus subtilis through suppression of carbon overflow by sodium citrate. Biotechnol Lett 27, 689–692 (2005). https://doi.org/10.1007/s10529-005-4686-1
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DOI: https://doi.org/10.1007/s10529-005-4686-1