Abstract
Of various yeasts tested in the conversion of fumaric to l-malic acid, Saccharomyces bayanus had the highest activity of fumarase. Cells permeabilized with 0.2% (w/v) CTAB for 5 min gave maximum enzyme activity. Under non-growth conditions, fumarase activity in the permeabilized cells was four times higher (271 U/g) than that of the intact cells (67 U/g). The proposed mathematical model for the batch production of l-malic acid was validated at different initial fumaric acid concentrations. The average conversion of fumaric acid was up to 82% and gave 21, 40, 83 and 175 mm l-malic acid from respectively, 25, 50, 100 and 210 mm fumaric acid.
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Abbreviations
- c fumaric acid :
-
fumaric acid concentration (mm)
- c malic acid :
-
l-malic acid concentration (mm)
- c yeast :
-
yeast cell concentration (g/l)
- K m1 :
-
Michaelis–Menten constant for fumaric acid (mm)
- K m2 :
-
Michaelis–Menten constant for l-malic acid (mm)
- K i :
-
inhibition constant (mm)
- r 1 :
-
rate of forward reaction (mmol/l min)
- r 2 :
-
rate of reverse reaction (mmol/l min)
- t :
-
time (min)
- T:
-
temperature (°C)
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Presečki, A.V., Vasić-Rački, Đ. Production of L-Malic Acid by Permeabilized Cells of Commercial Saccharomyces Sp. Strains. Biotechnol Lett 27, 1835–1839 (2005). https://doi.org/10.1007/s10529-005-3890-3
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DOI: https://doi.org/10.1007/s10529-005-3890-3