Abstract
Green tea polyphenol (GTP) together with dimethylsulphoxide (DMSO) were added to a freezing solution of osteoblastic cells (rat calvarial osteoblasts and human osteosarcoma cells) exposed to repeated freeze/thaw cycles (FTC) to induce oxidative stress. When cells were subjected to 3 FTCs, freezing medium containing 10% (v/v) DMSO and 500 μg GTP ml−1 significantly (p < 0.05) suppressed cell detachment and growth inhibition by over 63% and protected cell morphology. Furthermore, the alkaline phosphatase activity of osteoblastic cells was appreciably maintained after 2 and 3 FTCs in this mixture. Polyphenols may thus be of use as a cell cryopreservant and be advantageous in such fields as cell transplantation and tissue engineering.
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Han, DW., Kim, H.H., Lee, M.H. et al. Protection of osteoblastic cells from freeze/thaw cycle-induced oxidative stress by green tea polyphenol. Biotechnol Lett 27, 655–660 (2005). https://doi.org/10.1007/s10529-005-3677-6
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DOI: https://doi.org/10.1007/s10529-005-3677-6