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Fast screening target sites for RNA interference using a cell-free system

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Abstract

A fast and simple procedure to screen target sites for RNA interference by using RNA in a cell-free system of Hela cells, and then evaluating the efficiency by Northern blotting, is described. This procedure produces results with an identical reliability compared to those described previously but which are more time-consuming than this present method.

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Authors and Affiliations

  1. Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, Shanghai, 200031, China

    Huang Mei, Jia Fang-Jun, Li Chong-Yong, Li Yi-Ping & Guo Li-He

Authors
  1. Huang Mei
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  2. Jia Fang-Jun
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  3. Li Chong-Yong
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  4. Li Yi-Ping
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  5. Guo Li-He
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Corresponding author

Correspondence to Guo Li-He.

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Mei, H., Fang-Jun, J., Chong-Yong, L. et al. Fast screening target sites for RNA interference using a cell-free system. Biotechnol Lett 27, 619–621 (2005). https://doi.org/10.1007/s10529-005-3627-3

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  • DOI: https://doi.org/10.1007/s10529-005-3627-3

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