Skip to main content
SpringerLink
Log in

Direct FTIR Assay of Streptomycin in Agar

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Streptomycin titres in samples of agar media on which various species of streptomycetes were cultured were obtained by Fourier Transform Infra Red (FTIR) spectroscopy. Titres were directly comparable to those obtained by bioassay based on Bacillus subtilis inhibition. Analysis by this method could be used to facilitate the isolation of high level antibiotic-producing mutants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • M Beggs M Blok A Diels (1999) ArticleTitleThe high throughput screening infrastructure: the right tools for the task J.Biomol. Screen. 4 143–149 Occurrence Handle10.1177/108705719900400308 Occurrence Handle10838423

    Article  PubMed  Google Scholar 

  • PR Butler M Brown SG Oliver (1996) ArticleTitleImprovement of antibiotic titres from Streptomyces bacteria by interactive continuous selection Biotech. Bioeng. 49 185–196 Occurrence Handle10.1002/(SICI)1097-0290(19960120)49:2<185::AID-BIT7>3.0.CO;2-M

    Article  Google Scholar 

  • P Cunniff (1998) Official Method 962.14, Chapter 33,41--3, Association of Official Analytical Chemists (AOAC) Official Methods of Analysis EditionNumber16 NumberInSeries2 AOAC International Gaithersburg, MD, USA

    Google Scholar 

  • BG Harnulv BG Snygg (1972) ArticleTitleHeat-resistance of Bacillus subtilis spores at various water activities J. Appl. Bact. 35 615–624

    Google Scholar 

  • M Horie T Yoshida Y Kikuchi H Nakazawa (2001) ArticleTitleDetermination of streptomycin and dihydrostreptomycin in meat by liquid chromatography/mass spectrometry J. Food Hyg. Soc. Jpn. 42 374–378

    Google Scholar 

  • SC Hsu JL Lockwood (1975) ArticleTitlePowdered chitin agar as a selective medium for enumeration of Actinomycetes in water and soil Appl. Microbiol. 29 422–426 Occurrence Handle234719

    PubMed  Google Scholar 

  • IS Hunter (1999) Microbial synthesis of commercial products and strain improvement EMT El-Mansi CFA Bryce (Eds) Fermentation Microbiology and Biotechnology Taylor and Francis Ltd London 121–145

    Google Scholar 

  • DG Strauss (1974) ArticleTitleA fast comparison of IR-spectra for screening new antibiotics J. Antibiot. 27 805–808 Occurrence Handle4457529

    PubMed  Google Scholar 

  • DH Williams I Fleming (1989) Spectroscopic Methods in Organic Chemistry EditionNumber4 McGraw-Hill London

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemical Engineering, Loughborough University, LE11 3TU, Loughborough, Leicestershire, UK

    Richard J.L. Meanwell & Gilbert Shama

Authors
  1. Richard J.L. Meanwell
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gilbert Shama
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Gilbert Shama.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Meanwell, R.J., Shama, G. Direct FTIR Assay of Streptomycin in Agar. Biotechnol Lett 27, 1629–1631 (2005). https://doi.org/10.1007/s10529-005-2604-1

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-005-2604-1

Keywords

Search

Navigation