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The Use of Phase Inversion Temperature (PIT) Microemulsion Technology to Enhance Oil Utilisation during Streptomyces rimosus Fed-batch Fermentations to Produce Oxytetracycline

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Abstract

The use of a rapeseed oil emulsion feed, produced by a phase inversion temperature (PIT) process, produced more biomass, gave a 3-fold increase in oil utilisation and a higher oxytetracycline titre but a higher residual oil concentration when compared to a conventional fed-batch Streptomyces rimosus process fed with crude rapeseed oil. Importantly, microbial utilisation of the surfactant was confirmed for the first time.

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Authors and Affiliations

  1. Biochemical Engineering, Department of Chemical Engineering, The University of Birmingham, B15 2TT, Edgbaston, Birmingham, UK

    Panos A. Papapanagiotou, Alvin W. Nienow & Christopher J. Hewitt

  2. Pfizer Global Manufacturing, Global Manufacturing Services, Pfizer Inc., 235 East 42nd Street 685/6/11, New York, NY, 10017, USA

    Henry Quinn

  3. Cognis France, Fermentation Specialties, 185 av de Fontainebleau, F-77981, St. Fargeau-Ponthierry, France

    Jean-Pierre Molitor

Authors
  1. Panos A. Papapanagiotou
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  2. Henry Quinn
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  3. Jean-Pierre Molitor
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  4. Alvin W. Nienow
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  5. Christopher J. Hewitt
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Corresponding author

Correspondence to Christopher J. Hewitt.

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Papapanagiotou, P.A., Quinn, H., Molitor, JP. et al. The Use of Phase Inversion Temperature (PIT) Microemulsion Technology to Enhance Oil Utilisation during Streptomyces rimosus Fed-batch Fermentations to Produce Oxytetracycline. Biotechnol Lett 27, 1579–1585 (2005). https://doi.org/10.1007/s10529-005-2512-4

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  • DOI: https://doi.org/10.1007/s10529-005-2512-4

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