Abstract
Recent technological improvements have extended the application range of permittivity biomass measurements to bacterial fermentations in highly conductive media. With Lactobacillus casei, the effective biomass detection sensitivity of the FOGALE Biomass System is around 0.2 g/l (0.01 pF/cm). Fermentations growth kinetics of Lactobacillus casei can be recorded with good reproducibility and accuracy despite the high medium conductivity varying between 15 and 75 mS/cm, and the low cell concentration (<6 g/l).
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References
BC Blake-Coleman MR Calder RJG Carr SC Moody DJ Clarke (1984) ArticleTitleDirect monitoring of reactor biomass in fermentation control Trends Anal. Chem. 3 229–235 Occurrence Handle10.1016/0165-9936(84)87037-5
M Ciureanu W Levadoux S Goldstein (1997) ArticleTitleElectrical impedance studies on a culture of a newly discovered strain of Streptomyces Enzyme Microb. Technol. 21 441–449 Occurrence Handle10.1016/S0141-0229(97)00017-3
CL Davey DB Kell (1998a) ArticleTitleThe influence of electrode polarisation on dielectric spectra, with special reference to capacitive biomass measurements I. Quantifying the effects on electrode polarisation of factors likely to occur during fermentations Bioelectrochem. Bioenerg 46 91–103 Occurrence Handle10.1016/S0302-4598(98)00131-7
CL Davey DB Kell (1998b) ArticleTitleThe influence of electrode polarisation on dielectric spectra, with special reference to capacitive biomass measurements: (II) reduction in the contribution of electrode polarisation to dielectric spectra using a two-frequency method Bioelectrochem. Bioenerg. 46 105–114 Occurrence Handle10.1016/S0302-4598(98)00132-9
CL Davey W Penaloza DB Kell JN Hedger (1991) ArticleTitleReal-time monitoring of the accretion of Rhizopus oligosporus biomass during the solid-substrate tempe fermentation World J. Microbiol. Biotechnol. 7 248–259 Occurrence Handle10.1007/BF00328998
R Fehrenbach M Comberbach JO Petre (1992) ArticleTitleOn-line biomass monitoring by capacitance measurement J Biotechnol. 23 303–314 Occurrence Handle10.1016/0168-1656(92)90077-M Occurrence Handle1368248
CM Harris RW Todd SJ Bungart RW Lovitt JG Morris DB Kell (1987) ArticleTitleDielectric permittivity of microbial suspensions at radio-frequencies: a novel method for the real-time estimation of microbial biomass Enzyme Microb. Technol. 9 181–186 Occurrence Handle10.1016/0141-0229(87)90075-5
S Mas F Ossart C Ghommidh (2001) ArticleTitleOn-line size measurement of yeast aggregates using image analysis Biotechnol. Bioeng. 76 91–98 Occurrence Handle10.1002/bit.1148 Occurrence Handle11505378
HP Schwan (1957) ArticleTitleElectrical properties of tissue and cell suspensions Adv. Bio. Med. Phys. 5 147–209
HP Schwan (1963) Determination of biological impedances W. Nastuk (Eds) Physical Techniques in Biological Research NumberInSeriesvol. 6. Academic Press New York 323–407
NG Stoicheva CL Davey GH Markx DB Kell (1989) ArticleTitleDielectric spectroscopy: a rapid method for the determination of solvent biocompatibility during biotransformations Biocatalysis 2 245–255
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Arnoux, A., Preziosi-Belloy, L., Esteban, G. et al. Lactic Acid Bacteria Biomass Monitoring in Highly Conductive Media by Permittivity Measurements. Biotechnol Lett 27, 1551–1557 (2005). https://doi.org/10.1007/s10529-005-1781-2
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DOI: https://doi.org/10.1007/s10529-005-1781-2