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Expression of the Cercosporin Transporter, CFP, in Tobacco reduces Frog-eye Lesion Size

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Abstract

The cercosporin Major Facilitator Superfamily (MFS) transporter, CFP, under the control of the CaMV 35S promoter, was introduced into the Xanthi cultivar of tobacco by Agrobacterium-mediated transformation. CFP+ transgenic plants were physically indistinguishable from non-transgenic Xanthi and progressed normally through growth to seed set. Accumulation of CFP in the leaf membrane fraction of CFP+ transgenic plants was associated with decreased cercosporin phytotoxicity. Frog-eye leaf lesions on CFP+ transgenic plants infected with Cercospora nicotianae conidia were smaller but were similar in number to those on non-transgenic plants. We conclude that transgenic expression of CFP may have relevance for a disease control strategy in Cercospora-plant pathosystems where cercosporin is implicated in pathogen virulence.

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Abbreviations

ABC:

adenosine triphosphate binding cassette transporter

CaMV:

35S cauliflower mosaic virus 35S promoter

CFP:

cercosporin facilitator protein

FAD:

flavin adenine dinucleotide

GUS:

β-glucuronidase gene

MF:

major facilitator

MFS:

major facilitator superfamily

NT:

non transgenic plant

PCR:

polymerase chain reaction

PDX:

pyridoxine

PR:

pathogenicity related

R 0 :

original plant transformants

R 1 :

first generation plants resulting from selfing

T11, T17, T24, T25:

CFP R1 transgenic plants 11, 17, 24, 25

UV:

ultraviolet

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Authors and Affiliations

  1. Agricultural Research Service, U. S. Department of Agriculture, North Carolina State University, Raleigh, NC, 27695, USA

    Robert G. Upchurch & Mark S. Rose

  2. Department of Plant Pathology, North Carolina State University, Raleigh, NC, 27695, USA

    Robert G. Upchurch, Mark S. Rose & Mohamed Eweida

  3. Department of Botany, North Carolina State University, Raleigh, NC, 27695, USA

    Weineng Zuo

  4. Syngenta Biotechnology Institute, 3054 Cornwallis Rd. Research Triangle Park, NC, 27709-2257, USA

    Mark S. Rose

  5. Division of Cellular and Molecular Biology, Ontario Cancer Institute/Princess Margaret Hospital, 610 University Ave., M5G 2M9, Toronto, Ontario, Canada

    Mohamed Eweida

  6. TechWorld International Inc., 6537 Meadowbrook Court, West Chester, OH, 45069, USA

    Weineng Zuo

Authors
  1. Robert G. Upchurch
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  2. Mark S. Rose
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  3. Mohamed Eweida
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  4. Weineng Zuo
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Correspondence to Robert G. Upchurch.

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Upchurch, R.G., Rose, M.S., Eweida, M. et al. Expression of the Cercosporin Transporter, CFP, in Tobacco reduces Frog-eye Lesion Size. Biotechnol Lett 27, 1543–1550 (2005). https://doi.org/10.1007/s10529-005-1780-3

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  • DOI: https://doi.org/10.1007/s10529-005-1780-3

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