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Enhancing Cell Viability with Pulsating Flow in a Hollow Fiber Bioartificial Liver

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Abstract

A pulsating flow of medium was used to alleviate diffusion and transport limitations in a hollow fiber bioreactor containing a human hepatoblastoma cell line. The strategy is easy to implement but effective. The pulsating flow is introduced by a solenoid pinch valve at the outlet of the bioreactor and regulated by a timing circuit. In a permeability test, the system with pulsating flow had much less membrane fouling as compared to the control, a conventional hollow fiber unit. In hepatocyte culture test runs, the pulsating-flow bioreactor demonstrated the ability to maintain a higher cell viability. Histological sections indicated significantly smaller necrotic regions in the pulsating-flow bioreactor as compared to the conventional unit.

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Authors and Affiliations

  1. Applied Molecular Evolutions, 3520 Dunhill St., San Diego, CA, 92121, USA

    Diem T. Nguyen

  2. Vital Therapies Inc., 15222C Avenue of Science, San Diego, CA, 92128, USA

    John D. Brotherton

  3. Chemical Engineering Program, University of California, San Diego, La Jolla, CA, 92093, USA

    Pao C. Chau

Authors
  1. Diem T. Nguyen
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  2. John D. Brotherton
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  3. Pao C. Chau
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Correspondence to Pao C. Chau.

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Nguyen, D.T., Brotherton, J.D. & Chau, P.C. Enhancing Cell Viability with Pulsating Flow in a Hollow Fiber Bioartificial Liver. Biotechnol Lett 27, 1511–1516 (2005). https://doi.org/10.1007/s10529-005-1467-9

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  • DOI: https://doi.org/10.1007/s10529-005-1467-9

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