Skip to main content

Biotransformation of Isoeugenol to Vanillin by a Novel Strain of Bacillus fusiformis


A novel strain of Bacillus fusiformis, producing high amounts of vanillin from isoeugenol, was isolated from soil. Using 60% (v/v) isoeugenol as substrate and solvent and at pH 4.0, 37 °C and 180 rpm, vanillin was produced at 32.5 g l−1 over 72 h. The unused isoeugenol was reusable.

This is a preview of subscription content, access via your institution.


  • RE Buchanan NE Gibbons (Eds) (1986) Bergey’s Manual of Systematic Bacteriology EditionNumber9th Williams & Wilkins Baltimore 1104–1139

    Google Scholar 

  • YH Li ZH Sun P Zheng (2004) ArticleTitleDetermination of vanillin, eugenol and isoeugenol by RP-HPLC Chromatographia 60 709–713

    Google Scholar 

  • YH Li ZH Sun LQ Zhao Y Xu (2005) ArticleTitleBiotransformation of isoeugenol to vanillin by crude enzyme extracted from soybean Appl. Biochem. Biotechnol. 125 1–10

    Google Scholar 

  • J Overhage H Priefert J Rabenhorst A Steinbuchel (1999) ArticleTitleBiotransformation of eugenol to vanillin by a mutant of pseudomonas sp. Strain HR199 constructed by disruption of the vanillin dehydrogenase (vdh) gene Appl. Microbiol. Biotechnol. 52 820–828

    Google Scholar 

  • H Priefert J Babenhorst A Steinbuchel (2001) ArticleTitleBiotechnological production of vanillin Appl. Microbiol. Biotechnol. 56 296–314

    Google Scholar 

  • FG Priest M Goodfellow C Todd (1988) ArticleTitleA numerical classification of the genus Bacillus J. Gen. Microbiol. 134 1847–1882

    Google Scholar 

  • SR Rao GA Ravishankar (1999) ArticleTitleBiotransformation of isoeugenol to vanilla flavour metabolites and capsaicin in suspended and immobilized cell cultures of Capsicum frutescens: study of the influence of β-cyclodextrin and fungal elicitor Proc. Biochem. 35 341–348

    Google Scholar 

  • J Schrader MMW Etschmann D Sell JM Hilmer J Rabenhorst (2004) ArticleTitleApplied biocatalysis for the synthesis of natural flavour compounds current industrial processes and future prospects Biotechnol. Lett. 26 463–472

    Google Scholar 

  • E Shimoni T Baasov U Ravid Y Shoham (2003) ArticleTitleBiotransformations of propenylbenzenes by an Arthrobacter sp. and its t-anethole blocked mutants J. Biotechnol. 105 61–70

    Google Scholar 

  • E Shimoni U Ravid Y Shoham (2000) ArticleTitleIsolation of a Bacillus sp. capable of transforming isoeugenol to vanillin J. Biotechnol. 78 1–9

    Google Scholar 

  • Sun ZH (2002) A strain of Aspergillus niger and method for producing vanillic acid and vanillin by biotransformation. Chinese Patent CN02125561.X

  • NJ Walton A Narbad CB Faulds G Williamson (2000) ArticleTitleNovel approaches to biosynthesis of vanillin Curr. Opin. Biotech. 11 490–496

    Google Scholar 

  • Washisu Y, Tetsushi A, Hashimoto N, Kanisawa T (1993) Manufacture of vanillin and related compounds with Pseudomonas. Japanese Patent JP52279

Download references

Author information

Authors and Affiliations


Corresponding author

Correspondence to Zhi-Hao Sun.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Zhao, LQ., Sun, ZH., Zheng, P. et al. Biotransformation of Isoeugenol to Vanillin by a Novel Strain of Bacillus fusiformis. Biotechnol Lett 27, 1505–1509 (2005).

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI:


  • Bacillus fusiformis
  • Biotransformation
  • Isoeugenol
  • Isolation
  • Vanillin