Abstract
The steroidogenic factor (SF)-1 gene is one of a number of orphan nuclear receptors, which is a key transcriptional regulator in vertebrate reproduction. We have isolated the SF-1 homologue cDNA from the goldfish pituitary and designed primers for SF-1 on the basis of the highly conserved regions of various known SF-1 superfamily genes. SF-1 cDNA contained 1,948 nucleotides including an open reading frame predicted to encode a protein of 503 amino acids. The distribution pattern of SF-1 in a variety of tissues during sexual maturation in female goldfish was also examined by RT-PCR. Significant variations in the relative expression of SF-1 were observed in different tissues in immature and mature female goldfish. SF-1 transcript in pituitary was significantly higher than other tissues tested in immature and mature female goldfish. Lower expression of SF-1 was observed in the liver but was not detected in brain and ovary of the immature female goldfish. Presence of SF-1 was the predominant expression in the pituitary and brain of mature female goldfish. Also, in the mature female goldfish, a weak transcript was detected in liver and ovary. Interestingly, RT-PCR analysis revealed that the expression of SF-1 became higher in the brain and weaker in the liver in maturing female goldfish. Thus, SF-1 may be regulated in goldfish brain and/or liver. Thus is also tissue-specific distribution of SF-1 during sexual maturation in female goldfish.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
KM Barnhart PL Mellon (1994) ArticleTitleThe orphan nuclear receptor, Steroidogenic factor-1, regulates the glycoprotein hormone α-subunit gene in pituitary gonadotropes Mol. Endocrinol. 9 878–885 Occurrence Handle10.1210/me.8.7.878
C Chai W-K Chan (2000) ArticleTitleDevelopmental expression of a novel FTZ-F1 homologue, ff1b (NR5A4), in the zebrafish (Danio rerio) Mech. Develop. 91 421–426 Occurrence Handle10.1016/S0925-4773(99)00312-3
CY Choi HR Habibi (2003) ArticleTitleMolecular cloning of estrogen receptor α and expression pattern of estrogen receptor subtypes in male and female goldfish Mol. Cell Endocrinol. 204 169–177 Occurrence Handle10.1016/S0303-7207(02)00182-X Occurrence Handle12850291
L Galarneau J-F Pare D Allard D Hamei L Levesque JD Tugwood S Green L Belanger (1996) ArticleTitleThe α-1 fetoprotein locus is activated by a nuclear receptor of the Drosophila FTZ-F1 family Mol. Cell Biol. 16 3853–3865 Occurrence Handle8668203
L Galarneau R Drouin L Belanger (1998) ArticleTitleAssignment of the fetoprotein transcription factor gene (FTF) to human chromosome band 1q32.11 by in situ hybridization Cytogenet. Cell Genet. 82 269–270 Occurrence Handle10.1159/000015116 Occurrence Handle9858833
JV Hofsten I Jones J Karlsson P-E Olsson (2001) ArticleTitleDevelopmental expression patterns of FTZ-F1 homologues in zebrafish (Danio rerio) Gen. Comp. Endocrinol. 121 146–155 Occurrence Handle10.1006/gcen.2000.7582 Occurrence Handle11178880
S Honda K Morohashi M Nomura M Takeya M Kitajima T Omura (1993) ArticleTitleAd4BP regulating steroidogenic P-450 gene is a number of steroid hormone receptor superfamily J. Biol. Chem. 268 7498–7502
Y Ikeda WH Shen HA Ingraham KL Parker (1994) ArticleTitleDevelopmental expression of mouse steroidogenic factor-1, an essential regulator of the steroid hydroxylases Mol. Endocrinol. 8 654–662 Occurrence Handle10.1210/me.8.5.654 Occurrence Handle8058073
HA Ingraham DS Lala Y Ikeda X Luo WH Shen MW Nachtigal R Abbud JH Nilson KL Parker (1994) ArticleTitleThe nuclear receptor SF-1 acts multiple levels of the reproductive axis Genes Dev. 8 2302–2312 Occurrence Handle7958897
K Kawano I Miura K Morohashi M Takase M Nakamura (1998) ArticleTitleMolecular cloning and expression of the SF-1/Ad4BP gene in the frog Rana Rugosa. Gene 222 169–176 Occurrence Handle10.1016/S0378-1119(98)00498-3
T Kudo S Sutou (1997) ArticleTitleMolecular cloning of chicken FTZ-F1-related orphan receptors Gene 197 261–268 Occurrence Handle10.1016/S0378-1119(97)00270-9 Occurrence Handle9332374
D Liu Y Drean ParticleLe M Ekker F Xiong CL Hew (1997) ArticleTitleTeleost FTZ-F1 homolog and its splicing variant determine the expression of the salmon gonadotropin II beta subunit gene Mol. Endocrinol. 11 877–890 Occurrence Handle10.1210/me.11.7.877 Occurrence Handle9178748
Z Liu ER Simpson (1997) ArticleTitleSteroidogenic factor 1 (SF-1) and sp1 are required for regulation of bovine CYP11A gene expression in bovine luteal cells and adrenal Y1 cells Mol. Endocrinol. 11 127–137 Occurrence Handle10.1210/me.11.2.127 Occurrence Handle9013760
JP Lynch DS Lala JJ Peluso W Luo KL Parker BA White (1993) ArticleTitleSteroidogenic factor 1, an orphan nuclear receptor, regulates the expression of the rat aromatase gene in gonadal tissues Mol. Endocrinol. 7 776–786 Occurrence Handle10.1210/me.7.6.776 Occurrence Handle8395654
DJ Mangelsdorf C Thummel M Beato P Herrlich G Schutz K Umesono B Blumberg P Kastner M Mark P Chambon RM Evans (1995) ArticleTitleThe nuclear receptor superfamily: the second decade Cell 83 835–839 Occurrence Handle10.1016/0092-8674(95)90199-X Occurrence Handle8521507
T Nakajima M Takase M Nakamura (2000) ArticleTitleTwo isoforms of FTZ-F1 messenger RNA: molecular cloning and their expression in the frog testis Gene 248 203–212 Occurrence Handle10.1016/S0378-1119(00)00119-0 Occurrence Handle10806365
NJ Proudfoot GG Brownlee (1976) ArticleTitle3’ Non-coding region sequences in eukaryotic messenger RNA Nature 263 211–214 Occurrence Handle10.1038/263211a0 Occurrence Handle822353
C Smith MJ Smith AH Sinclair (1999) ArticleTitleExpression of chicken steroidogenic factor-1 during gonadal sex differentiation Gen. Comp. Endocrinol. 113 187–196 Occurrence Handle10.1006/gcen.1998.7214 Occurrence Handle10082621
L Tora J White C Brou D Tasset N Webster E Scheer P Chambon (1989) ArticleTitleThe human estrogen receptor has two independent nonacidic transcriptional activation functions Cell 59 477–487 Occurrence Handle10.1016/0092-8674(89)90031-7 Occurrence Handle2805068
LH Wang SY Tsai RG Cook WG Beattie MJ Tsai BW O’Malley (1989) ArticleTitleCOUP transcription factor is a member of the steroid receptor superfamily Nature (London) 340 163–166 Occurrence Handle10.1038/340163a0
M Watanabe M Tanaka D Kobayashi Y Yoshiura Y Oba Y Nagahama (1999) ArticleTitleMedaka (Oryzias latipes) FTZ-F1 potentially regulates the transcription of P-450 aromatase in ovarian follicles. cDNA cloning and functional characterization Mol. Cell Endocrinol. 149 221–228 Occurrence Handle10.1016/S0303-7207(99)00006-4 Occurrence Handle10375033
M Wong MS Ramayya GP Chrousos PH Driggers KL Parker (1996) ArticleTitleCloning and sequence analysis of the human gene encoding steroidogenic factor-1 J. Mol. Endocrinol. 17 139–147 Occurrence Handle10.1677/jme.0.0170139 Occurrence Handle8938589
Y Yu W Li K Su M Yussa W Han N Perrimon L Pick (1997) ArticleTitleThe nuclear receptor Ftz-F1 is a cofactor for the Drosophila homeodomain protein Ftz Nature 385 552–555 Occurrence Handle10.1038/385552a0 Occurrence Handle9020364
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Choi, C.Y., Habibi, H.R. Molecular Cloning and Tissue Distribution of SF-1-related Orphan Receptors During Sexual Maturation in Female Goldfish. Biotechnol Lett 27, 1283–1290 (2005). https://doi.org/10.1007/s10529-005-0220-8
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10529-005-0220-8