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Inhibition of Human UDP-Glucose Dehydrogenase Expression Using siRNA Expression Vector in Breast Cancer Cells

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Abstract

UDP-glucose dehydrogenase (UGDH) catalyzes two oxidations of UDP-glucose to yield UDP-glucuronic acid. Pathological over-production of extracellular matrix components may be linked to the availability of UDP-glucuronic acid, therefore UGDH is a potential therapeutic target. RNA interference (RNAi) has been adapted to knock down the expression of human UGDH. A UGDH siRNA plasmid was constructed using a pRNA-U6.1/Neo vector and transfected into breast cancer cells, ZR-75-1, with an efficiency of up to 50%. Western blot analysis showed that the UGDH expression was efficiently knocked down at protein levels by RNAi in ZR-75-1 cells.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, University of Ulsan College of Medicine, 388-1 Poongnap-dong, 138-736, Seoul, Songpa-gu, Korea

    Jae-Wan Huh, Myung-Min Choi, Seung-Ju Yang & Sung-Woo Cho

  2. Department of Anatomy and Cell Biology, University of Ulsan College of Medicine, 388-1 Poongnap-dong, 138-736, Seoul, Songpa-gu, Korea

    Seung Yong Yoon

  3. Department of Genetic Engineering, Division of Life Sciences, Hallym University, 200-702, Chunchon, Korea

    Soo Young Choi

Authors
  1. Jae-Wan Huh
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  2. Myung-Min Choi
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  3. Seung-Ju Yang
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  4. Seung Yong Yoon
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  5. Soo Young Choi
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  6. Sung-Woo Cho
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Correspondence to Sung-Woo Cho.

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Huh, JW., Choi, MM., Yang, SJ. et al. Inhibition of Human UDP-Glucose Dehydrogenase Expression Using siRNA Expression Vector in Breast Cancer Cells. Biotechnol Lett 27, 1229–1232 (2005). https://doi.org/10.1007/s10529-005-0022-z

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  • DOI: https://doi.org/10.1007/s10529-005-0022-z

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