Abstract
A rapid molecular approach was developed for the initial identification of Lactobacillus acidophilus strains which are difficult to identify using a single biochemical test. The 16S–23S rRNA intergenic spacer regions and flanking 23S rRNA genes of 19 strains of lactobacilli were amplified and the nucleotide sequences and restriction site polymorphisms were analyzed. AluI was the most useful of the restriction enzymes analyzed and produced reproducible digestion profiles in the L. helveticus, L. plantarum, and L. casei groups, as well as in L. acidophilus. This restriction fragment length polymorphism method may be useful for the identification of L. acidophilus strains in dairy products.
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References
F Berthier SD Ehrlich (1998) ArticleTitleRapid species identification within two groups of closely related lactobacilli using PCR primers that target the 16S/23S rRNA spacer region FEMS Microbiol. Lett 161 97–106 Occurrence Handle10.1016/S0378-1097(98)00055-X Occurrence Handle9561736
F Bringel M Curk J Hubert (1996) ArticleTitleCharacterization of lactobacilli by southern-type hybridization with a Lactobacillus plantarum pyrDFE probe Int. J. Syst. Bacteriol 46 588–594 Occurrence Handle8934911
PS Cocconcelli MG Parisi L Senini V Bottazzi (1997) ArticleTitleUse of RAPD and 16S rDNA sequencing for the study of Lactobacillus population dynamics in natural whey culture Lett. Appl. Microbiol 25 8–12 Occurrence Handle10.1046/j.1472-765X.1997.00061.x Occurrence Handle9248073
M Jensen N Straus (1993) ArticleTitleEffect of PCR conditions on the formation of heteroduplex and single-strained DNA products in the amplification of bacterial ribosomal DNA spacer regions PCR Methods Appl 3 186–194 Occurrence Handle8118401
CN Rachman P Kabadjova H Prévost X Dousset (2003) ArticleTitleIdentification of Lactobacillus alimentarius and Lactobacillus farciminis with 16S–23S rDNA intergenic spacer region polymorphism and PCR amplification using species-specific oligonucleotide J. Appl. Microbiol 95 1207–1216 Occurrence Handle10.1046/j.1365-2672.2003.02117.x Occurrence Handle14632993
D Roy P Ward D Vincent F Mondou (2000) ArticleTitleMolecular identification of potentially probiotic lactobacilli Curr. Microbiol 40 40–46 Occurrence Handle10.1007/s002849910008 Occurrence Handle10568802
D Roy S Sirois D Vincent (2001) ArticleTitleMolecular discrimination of lactobacilli used as starter and probiotic cultures by amplified ribosomal DNA restriction analysis Curr. Microbiol 42 282–289 Occurrence Handle11178730
S Salminen E Isolauri E Salminen (1996) ArticleTitleClinical uses of probiotics for stabilizing the gut mucosal barrier: successful strains and future challenges Antonie Van Leeuuwehoek 70 347–358 Occurrence Handle10.1007/BF00395941
ME Sanders (1993) ArticleTitleSummary of conclusions from a consensus panel of experts on health attributes of lactic cultures: significance of fluid milk products containing cultures J. Dairy Sci 76 1819–1828 Occurrence Handle8345120
JM Simpson VJ McCracken HR Gaskins R Mackie (2000) ArticleTitleDenaturing gradient gel electrophoresis analysis of 16S ribosomal DNA amplicons to monitor changes in fecal bacterial populations of weaning pigs after introduction of Lactobacillus reuteri strain MM53 Appl. Environ. Microbiol 66 4705–4714 Occurrence Handle10.1128/AEM.66.11.4705-4714.2000 Occurrence Handle11055913
YL Song N Kato CX Liu Y Matsunmiya H Kato K Watanabe (2000) ArticleTitleRapid identification of 11 human intestinal Lactobacillus species by multiplex PCR assays using group- and species-specific primers derived from the 16S–23S rRNA intergenic spacer region and its flanking 23S rRNA FEMS Microbiol. Lett 187 167–173 Occurrence Handle10.1016/S0378-1097(00)00196-8 Occurrence Handle10856652
LR Steenson TR Klaenhammer (1985) ArticleTitleStreptococcus cremoris M12R transconjugants carrying the conjugal plasmid pTR2030 are insensitive to attack by lytic bacteriophages Appl. Environ. Microbiol 50 851–853 Occurrence Handle3002270
A Tilsala-Timisjarvi T Alatossava (2001) ArticleTitleCharacterization of the 16S–23S and 23S–5S rRNA intergenic spacer regions of dairy propionibacteria and their identification with species-specific primers by PCR Int. J. Food Microbiol 68 45–52 Occurrence Handle10.1016/S0168-1605(01)00462-7 Occurrence Handle11545219
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Han, KS., Kim, Y., Choi, S. et al. Rapid Identification of Lactobacillus acidophilus by Restriction Analysis of the 16S–23S rRNA Intergenic Spacer Region and Flanking 23S rRNA Gene. Biotechnol Lett 27, 1183–1188 (2005). https://doi.org/10.1007/s10529-005-0014-z
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DOI: https://doi.org/10.1007/s10529-005-0014-z