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Enhancement of photorespiration in immobilized Chlamydomonas reinhardtii cells

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Abstract

Immobilization of Chlamydomonas reinhardtii in alginate increases its photorespiration rate. In the immobilized cells, the photorespiratory enzyme, phosphoglycolate phosphatase, was 75% higher than in freely suspended cells. Thus, the immobilized cells produced glycolate at twice the rate than in freely suspended cells when treated with aminooxyacetate (a transaminase inhibitor). With immobilized cells in a batch reactor, 270 μmol glycolate mg−1 Chl was produced after 12 h.

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Authors and Affiliations

  1. Departamento de Química y CC.MM (Area de Bioquímica), Facultad de Ciencias Experimentales, Campus El Carmen, Universidad de Huelva, 21017, Huelva, Spain

    I. Garbayo, E. Forján, A. Salguero, M. Cuaresma & C. Vílchez

  2. Departamento de Bioquímica Vegetal y Biología Molecular, Facultad de Química, Universidad de Sevilla, c/ Profesor García González 1, Sevilla, 41071, Spain

    J.M. Vega

Authors
  1. I. Garbayo
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  2. E. Forján
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  3. A. Salguero
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  4. M. Cuaresma
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  5. J.M. Vega
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  6. C. Vílchez
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Corresponding author

Correspondence to I. Garbayo.

Additional information

Revisions requested 27 October 2004; Revisions received 13 December 2004

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Garbayo, I., Forján, E., Salguero, A. et al. Enhancement of photorespiration in immobilized Chlamydomonas reinhardtii cells. Biotechnol Lett 27, 265–267 (2005). https://doi.org/10.1007/s10529-004-8352-9

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  • DOI: https://doi.org/10.1007/s10529-004-8352-9

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