Abstract
A fusion thymosin α1-soluble B lymphocyte stimulator (TMα1–cBLyS) gene was generated to engineer a bifunctional lymphokine, which was then over-produced in Escherichia coli. The molecular weight of the expressed fusion protein was approximately 28 kDa. After being purified by Ni-NTA affinity column, the fusion protein had full activity of BLyS with a slightly higher immunological action than synthetic TMα1. Because TMα1 regulates the cellular immune response and cBLyS amplifies the humoral response, this bifunctional lymphokine could be useful in the treatment of various immunodeficiency syndromes and serve as an immunomodulator to enhance the host’s response to vaccination.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
M Brodelius A Lundgren P Mercke PE Brodelius (2002) ArticleTitleFusion of farnesyldiphosphate synthase and epi-aristolochene synthase, a sesquiterpene cyclase involved in capsidiol biosynthesis in Nicotiana tabacum Eur. J. Biochem. 269 3570–3577 Occurrence Handle10.1046/j.1432-1033.2002.03044.x
E Garaci F Pica G Rasi C Favalli (2000) ArticleTitleThymosin alpha 1 in the treatment of cancer: from basic research to clinical application Int. J. Immunopharmacol. 22 1067–1076 Occurrence Handle10.1016/S0192-0561(00)00075-8
AL Goldstein TL Low M McAdoo J McClure GB Thurman J Rossio CY Lai D Chang SS Wang C Harvey AH Ramel J Meienhofer (1977) ArticleTitleThymosin alpha1: isolation and sequence analysis of an immunologically active thymic polypeptide Proc. Natl. Acad. Sci. USA 74 725–729
JA Gross J Johnston S Mudri R Enselman SR Dillon K Madden W Xu J Parrish-Novak D Foster C Lofton-Day M Moore A Littau A Grossman H Haugen K Foley H Blumberg K Harrison W Kindsvogel CH Clegg (2000) ArticleTitleTACI and BCMA are receptors for a TNF homologue implicated in B-cell autoimmune disease Nature 404(6781) 995–999 Occurrence Handle10.1038/35010115
SD Khare I Sarosi XZ Xia S McCabe K Miner I Solovyev N Hawkins M Kelley D Chang G Van L Ross J Delaney L Wang D Lacey WJ Boyle H Hsu (2000) ArticleTitleSevere B cell hyperplasia and autoimmune disease in TALL-1 transgenic mice Proc. Natl. Acad. Sci. USA 97 3370–3375 Occurrence Handle10.1073/pnas.050580697
SA Marsters M Yan RM Pitti PE Haas VM Dixit A Ashkenazi (2000) ArticleTitleInteraction of the TNF homologues BLyS and APRIL with the TNF receptor homologues BCMA and TACI Curr. Biol. 10 785–788 Occurrence Handle10.1016/S0960-9822(00)00566-2
PA Moore O Belvedere A Orr K Pieri DW LaFleur P Feng D Soppet M Charters R Gentz D Parmelee Y Li O Galperina J Giri V Roschke B Nardelli J Carrell S Sosnovtseva W Greenfield SM Ruben HS Olsen J Fikes DM Hilbert (1999) ArticleTitleBLyS: member of the tumor necrosis factor family and B lymphocyte stimulator Science 285 260–263 Occurrence Handle1:CAS:528:DyaK1MXks1Sju7g%3D Occurrence Handle10398604
N Sattar M Mihelic MS Shekhani M Hartleb K Folkers W Voelter (1991) ArticleTitleA modified E-rosette assay as a semi-empirical tool in search of new T lymphocyte stimulants Immunol. Lett. 27 221–223 Occurrence Handle10.1016/0165-2478(91)90155-4
P Schneider F MacKay V Steiner K Hofmann JL Bodmer N Holler C Ambrose P Lawton S Bixler H Acha-Orbea D Valmori P Romero C Werner-Favre RH Zubler JL Browning J Tschopp (1999) ArticleTitleBAFF, a novel ligand of the tumor necrosis factor family, stimulates B cell growth J. Exp. Med. 189 1747–1756 Occurrence Handle10.1084/jem.189.11.1747
JS Thompson P Schneider SL Kalled L Wang EA Lefevre TG Cachero F MacKay SA Bixler M Zafari ZY Liu SA Woodcock F Qian M Batten C Madry Y Richard CD Benjamin JL Browning A Tsapis J Tschopp C Ambrose (2000) ArticleTitleBAFF binds to the tumor necrosis factor receptor-like molecule B cell maturation antigen and is important for maintaining the peripheral B cell population J. Exp. Med. 192 129–135 Occurrence Handle10.1084/jem.192.1.129
RY Tian YH Huang Y Chen W Han Y Yu H Liu ZH Weng SL Yang Y Gong (2004) ArticleTitleExpression and identification of TACI/BCMA-IgG1 Fc fusion proteins in mammalian cells Acta Zool. Sin. 50 401–407
M Yan SA Marsters IS Grewal H Wang A Ashkenazi VM Dixit (2000) ArticleTitleIdentification of a receptor for BLyS demonstrates a crucial role in humoral immunity Nat. Immunol. 1 37–41 Occurrence Handle10.1038/76889
Author information
Authors and Affiliations
Corresponding authors
Additional information
Revisions requested 15 October 2004; Revisions received 26 November 2004;
Rights and permissions
About this article
Cite this article
Shen, Q., Tian, R., Ma, W. et al. Construction and expression of a new fusion protein, thymosin α1–cBLyS, E. coli. Biotechnol Lett 27, 143–148 (2005). https://doi.org/10.1007/s10529-004-7333-3
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s10529-004-7333-3