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Pest and disease resistance enhanced by heterologous suppression of a Nicotiana plumbaginifolia cytochrome P450 gene

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Abstract

The functional role of theNicotiana plumbaginifolia cytochrome P450 gene CYP72A2 was investigated in transgenic plants. N. tabacum plants transformed with a sense or antisense CYP72A2 construct exhibited diminished heights, branched stems, smaller leaves and deformed flowers. Western blot analysis revealed reduced levels of a 58 kDa protein corresponding to CYP72A2, suggesting that the CYP72A2 homolog was suppressed in the sense and antisense plants. Transgenic plants had increased resistance to Manduca sexta larvae that consumed about 35 to 90 less of transgenic versus control leaves. A virulent strain of Pseudomonas syringae pv. tabaci induced a disease-limiting response followed by a delayed and decreased development of disease symptoms in the transgenics. CYP72A2 gene mediated resistance suggests that the plant-pest or -pathogen interactions may have been modified by changes in bioactive metabolite pools.

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References

  • A Aharoni AP Giri S Deuerlein F Griepink Kogel W-J De FWA Verstappen HA Verhoeven MA Jongsma W Schwab HJ Bouwmeeester (2003) ArticleTitleTerpenoid metabolism in wild-type and transgenic Arabidopsis plants Plant Cell 15 2866–2884

    Google Scholar 

  • S Bak CE Olsen BA Halkier BL Moller (2000) ArticleTitleTransgenic tobacco and Arabidopsis plants expressing the two multifunctional sorghum cytochrome P450 enzymes, CYP79A1 and CYP71E1, are cyanogenic and accumulate metabolites derived from intermediates in dhurrin biosynthesis Plant Physiol. 123 1437–1448

    Google Scholar 

  • G Bartoszewski CV Mujer K Niemirowicz-Szczytt AC Smigocki (2002) ArticleTitleCloning of a wound inducible Lycopersicon esculentum cytochrome P450 gene and lack of regeneration of transgenic plants with sense or antisense constructs J. Amer. Soc. Hort. Sci. 127 535–539

    Google Scholar 

  • OD Dhingra JB Sinclair (1995) Basic Plant Pathology Methods CRC Press Boca Raton, FL 354

    Google Scholar 

  • K Feldmann (2001) ArticleTitleCytochrome P450s as genes for crop improvement Curr. Opin. Plant Biol. 4 162–167

    Google Scholar 

  • S Imler G Schroder B St-Pierre NP Crouch M Hotze J Schmidt D Strack U Matern J Schroder (2000) ArticleTitleIndole alkaloid biosynthesis in Catharanthus roseaus: new enzyme activities and identification of cytochrome P450 CPY72A1 as secologanin synthase Plant J. 24 797–804

    Google Scholar 

  • T Ito EM Meyerowitz (2000) ArticleTitleOverexpression of a gene encoding a cytochrome P450, CYP78A9, induces large and seedless fruit in Arabidopsis Plant Cell 12 1541–1550

    Google Scholar 

  • G-T Kim H Tsukaya Y Saito H Uchimiya (1999) ArticleTitleChanges in the shapes of leaves and flowers upon overexpresssion of cytochrome P450 in Arabidopsis Proc. Nat. Acad. Sci. USA 96 9433–9437

    Google Scholar 

  • CV Mujer AC Smigocki (2001) ArticleTitleCytokinin- and wound-inducible cytochrome P450 from Nicotiana plumbaginifolia Physiol. Plant. 111 172–181

    Google Scholar 

  • AC Smigocki S Heu I McCanna C Wozniak G Buta (1997) Insecticidal compounds induced by regulated overproduction of cytokinins in transgenic plants N Carozzi M Koziel (Eds) Advances in Insect Control: The Role of Transgenic Plants Taylor and Francis Inc. Bristol, PA 225–236

    Google Scholar 

  • D Takemoto M Hyashi N Doke M Nishimura K Kawakita (1999) ArticleTitleMolecular cloning of a defense-response-related cytochrome P450 gene from tobacco Plant Cell Physiol. 40 1232–1242

    Google Scholar 

  • der Meer IM Van ME Stam Tunen AJ van JNM Mol AR Stuitje (1992) ArticleTitleAntisense inhibition of flavonoid biosynthesis in petunia anthers results in male sterlility Plant Cell 4 253–262

    Google Scholar 

  • E Wang R Wang J DeParasis JH Laughrin S Gan GJ Wagner (2001) ArticleTitleSuppression of P450 hydroxylase gene in plant trichome glands enhances natural-product-based aphid resistance Nat. Biotechnol. 19 371–374

    Google Scholar 

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Authors and Affiliations

  1. Molecular Plant Pathology Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD, 20705, USA

    Ann C. Smigocki & Dennis Wilson

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  1. Ann C. Smigocki
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  2. Dennis Wilson
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Correspondence to Ann C. Smigocki.

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Smigocki, A.C., Wilson, D. Pest and disease resistance enhanced by heterologous suppression of a Nicotiana plumbaginifolia cytochrome P450 gene. Biotechnol Lett 26, 1809–1814 (2004). https://doi.org/10.1007/s10529-004-4615-8

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  • DOI: https://doi.org/10.1007/s10529-004-4615-8

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