Abstract
Nitrate at 70 m gave the highest biomass of Camptotheca acuminata in suspension culture in MS medium, but a NH4 +/NO3 − molar ratio of 5:1 (giving a total of 40 m N) gave the maximum camptothecin yield. A two-stage flask culture system was established to improve culture efficiency; cell dry weight, camptothecin content and yield was increased by 30%, 280% and 340%, respectively when compared with those of control, reaching up to 36 g l−1, 0.36 mg g−1, and 12.8 mg l−1, respectively.
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Pan, Xw., Xu, Hh., liu, X. et al. Improvement of growth and camptothecin yield by altering nitrogen source supply in cell suspension cultures of Camptotheca acuminata . Biotechnology Letters 26, 1745–1748 (2004). https://doi.org/10.1007/s10529-004-4580-2
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DOI: https://doi.org/10.1007/s10529-004-4580-2