Skip to main content

Adventitious root growth and ginsenoside accumulation in Panax ginseng cultures as affected by methyl jasmonate

Abstract

Adventitious roots of ginseng were treated with methyl jasmonate (MJ) up to 150μm and cultured for 40days. Up to 100μm MJ inhibited the root growth but increase ginsenoside accumulation. In a two-stage bioreactor culture, total ginsenosides, after elicitation with 100 μm MJ peaked after 10days at 48mgg−1 dry wt and then dropped sharply. Of the two groups of ginsenosides (Rb and Rg), higher amounts of Rb accumulated in the adventitious roots.

This is a preview of subscription content, access via your institution.

References

  • H Aoyagi Y Kobayashi K Yamada M Yokoyama K Kusakari H Tanaka (2001) ArticleTitleEfficient production of saikosaponins in Bupleurum falcatum root fragments combined with signal transducers Appl. Microbiol. Biotechnol. 57 482–488

    Google Scholar 

  • EJ Hahn YS Kim KW Yu CS Jeong KY Paek (2003) ArticleTitleAdventitious root cultures of Panax ginseng C.A. Meyer and ginsenoside production through large-scale bioreactor system J. Plant Biotech. 5 1–6

    Google Scholar 

  • REB Ketchum DM Gibson RB Croteau ML Shuler (1999) ArticleTitleThe kinetics of taxoid accumulation in cell suspension cultures of Taxus following elicitation with methyl jasmonate Biotechnol. Bioeng. 62 97–105

    Google Scholar 

  • J Palazon RM Cusido M Bonfill A Mallol E Moyano C Morales MT Pinol (2003) ArticleTitleElicitation of different Panax ginseng transformed root phenotypes for an improved ginsenoside production Plant Physiol. Biochem. 41 1019–1025

    Google Scholar 

  • YH Shin SC Kim JW Han JY Oh OM Jeong DH Shin SY Nah (1997) ArticleTitleGinseng protopanaxatriol saponins but not protopanaxadiol saponins inhibit spontaneous motility of intestine Koryo Insam Hakheochi. 21 35–38

    Google Scholar 

  • J Wu JJ Zhong (1999) ArticleTitleProduction of ginseng and its bioactive components in plant cell culture: current technological and applied aspects, J Biotechnol. 68 89–99

    Google Scholar 

  • KW Yu W Gao EJ Hahn KY Paek (2002) ArticleTitleJasmonic acid improving ginsenoside accumulation in adventitious root culture of Panax ginseng C.A. Meyer Biochem. Eng. J. 11 211–215

    Google Scholar 

  • Y Yukimune H Tabata Y Higashi Y Hara (1996) ArticleTitleMethyl jasmonate-induced overproduction of paclitaxel and baccatin III in Taxus cell suspension cultures Nature Biotechnol. 14 1129–1132

    Google Scholar 

  • GZ Zheng CR Yang (1994) Sanchi (Panax notoginseng) Biology and Applications Science Press Beijing

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Kee-yoeup Paek.

Additional information

Revisions requested; 2 July 2004; Revisions received 30 June 2004; 3 September 2004

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Kim, Ys., Hahn, Ej., Murthy, H.N. et al. Adventitious root growth and ginsenoside accumulation in Panax ginseng cultures as affected by methyl jasmonate. Biotechnol Lett 26, 1619–1622 (2004). https://doi.org/10.1007/s10529-004-3183-2

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-004-3183-2

Keywords

  • adventitious roots
  • bioreactor cultures
  • ginseng
  • ginsenosides